PXD058971

PXD058971 is an original dataset announced via ProteomeXchange.

Title	Multi-omics Analysis Shed Light on the Extracellular Role of PCMT1
Description	Proteins are constantly damaged by intracellular and extracellular factors, particularly under stress conditions, leading to an accumulation of modified proteins like isoaspartate (isoD), which is linked to aging and various diseases. The repair enzyme PCMT1 (Protein-L-isoaspartate O-methyltransferase) specifically restores isoD residues to aspartate, preventing abnormal protein functions. Despite its importance, its role in non-neural tissues remain underexplored. This study employed isoD-proteomics, global proteomics, and transcriptomics on pcmt1 knockout (KO) and wild-type (WT) mice, revealing significant gene and protein changes in various KO tissues, enriched in extracellular and membrane-related categories, highlighting genome-proteome correlations. IsoD-carrying proteins were increased in KO tissues, predominantly consisting of long-lived proteins. Additionally, proteomic analysis of PCMT1-overexpressing cells identified interacting proteins mainly in extracellular and membrane-related categories. These findings revealed both tissue-specific and shared roles of PCMT1, emphasizing its importance in maintaining protein integrity under physiological and stress conditions, while also uncovering an unexpected extracellular function of PCMT1.
HostingRepository	PRIDE
AnnounceDate	2025-06-16
AnnouncementXML	Submission_2025-06-15_16:21:50.025.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JIE WANG
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-18 00:35:19	ID requested
⏵ 1	2025-06-15 16:21:50	announced

10.1021/acs.jproteome.4c01111;

Wang J, Xia J, Su J, Cao Z, Yang W, Zhang P, Xu Y, Multi-omics Analysis Sheds Light on the Extracellular Role of PCMT1. J Proteome Res, 24(6):2832-2845(2025) [pubmed]

submitter keyword: PCMT1

Multi-omics

Isoaspartate

Extracellular

Stress condition

Yingjie Xu
contact affiliation	Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, P.R. China
contact email	xuyingjie@shsmu.edu.cn
lab head
JIE WANG
contact affiliation	Shanghai Jiao Tong University School of Medicine
contact email	wangjie624@sjtu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD058971

PRIDE project URI

• PRIDE
  1. PXD058971
     1. Label: PRIDE project
     2. Name: Multi-omics Analysis Shed Light on the Extracellular Role of PCMT1