PXD058913 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comprehensive analysis of the proteome of S. cerevisiae wild-type and pdr5 cells in response to Bisphenol A (BPA) exposure |
Description | Bisphenol A, an endocrine-disrupting compound, is used widely in the industrial production of plastic products. Despite increasing concerns about its harmful effects on human health, animals and the environment, the use of BPA has been banned only in infant products and its effects on cellular processes are not fully understood. To investigate the impact of BPA on eukaryotic cells, we analyzed proteome changes of wild-type and PDR5-deleted S. cerevisiae strains exposed to different doses of BPA using sample multiplexing-based quantitative mass spectrometry. We found that the ABC multidrug transporter Pdr5 plays an important role in protecting yeast cells from BPA toxicity, with its absence significantly sensitizes cells to BPA. BPA inhibited yeast growth in a dose-dependent manner, with a more pronounced effect in PDR5-deleted cells. Proteomic analysis revealed that BPA induces dose-dependent widespread changes in protein abundance, including the up-regulation of metabolic pathways such as arginine biosynthesis and the down-regulation of mitochondrial proteins. Additionally, we observed markers of cellular stress induced by BPA by identifying multiple stress-induced proteins up-regulated by this compound. As cellular processes affected by BPA have been shown to be evolutionary conserved, these insights can advance our understanding of BPA’s cellular impact and its broader effects on human health. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_06:04:47.274.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joao Paulo |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue |
Instrument | Orbitrap Astral |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-12-16 10:00:40 | ID requested | |
⏵ 1 | 2025-05-07 06:04:48 | announced | |
Publication List
Keyword List
submitter keyword: Bisphenol A (BPA), TMT proteomics, Stress, Proteome,S. cerevisiae |
Contact List
Joao A. Paulo |
contact affiliation | Joao A. Paulo Dept. of Cell Biology Harvard Medical School Boston, MA, USA |
contact email | joao_paulo@hms.harvard.edu |
lab head | |
Joao Paulo |
contact affiliation | Harvard Medical School |
contact email | joao_paulo@post.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058913
- Label: PRIDE project
- Name: Comprehensive analysis of the proteome of S. cerevisiae wild-type and pdr5 cells in response to Bisphenol A (BPA) exposure