PXD058901

PXD058901 is an original dataset announced via ProteomeXchange.

Title	Analysis of reduction capability of GrxS12 on SufB and its cysteine residues
Description	To elucidate the reduction of GrxS12 on SufB, an in vitro reducing system was utilized. The mass spectrometry analysis of the GrxS12-reduced SufB samples identified and quantified a large number of SufB peptides containing a total of eight Cys residues. The Cys-containing peptides of SufB exhibited a notable reduction in relative intensity when reduced by GrxS12C34S, in comparison to those reduced by the normal GrxS12. When these peptides were reduced by GrxS12C92S, the identities of GSSG-oxidized peptides were obviously reduced, whereas those of H2O2-oxidized peptides remained unaltered. The oxidation level of SufB under GSSG and H2O2 treatments was quantified by mass spectrometry. It was observed that four Cys sites (Cys108, Cys176, Cys415, and Cys477) of SufB exhibited sulfenation, sulfination, and sulfonation at varying degrees. With regard to Cys108 and Cys415, sulfenation represents the predominant form of oxidation, whereas the majority of Cys176 and Cys477 underwent sulfonation. Importantly, the level of oxidation at Cys415 was significantly higher than that observed at other three Cys sites.
HostingRepository	PRIDE
AnnounceDate	2026-01-16
AnnouncementXML	Submission_2026-01-15_19:52:35.377.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanshuang Liu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	dihydroxylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-16 03:19:41	ID requested
⏵ 1	2026-01-15 19:52:35	announced

10.1111/TPJ.70666;

submitter keyword: GrxS12, SufB, reduction of cysteine residue

Shaojun Dai
contact affiliation	Shanghai Normal University,Shanghai,China
contact email	daishaojun@shnu.edu.cn
lab head
Yanshuang Liu
contact affiliation	Shanghai Normal University
contact email	920662600@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058901
     1. Label: PRIDE project
     2. Name: Analysis of reduction capability of GrxS12 on SufB and its cysteine residues