PXD058897

PXD058897 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	An updated proteomic analysis of Drosophila hemolymph after bacterial infection
Description	Using an in-depth Mass Spectrometry based proteomics approach, we provide a comprehensive characterization of the hemolymphatic proteome of adult flies upon bacterial infection. We detected and quantified changes in abundance of several known immune regulators and effectors, including multiple antimicrobial peptides, peptidoglycan-binding proteins and serine proteases. Comparison to previously published transcriptomic analyses reveals a partial overlap with our dataset, indicating that many proteins released into the hemolymph upon infection may not be regulated at the transcript level. Among them, we identify a set of muscle-derived proteins released into the hemolymph upon infection. Finally, our analysis reveals that infection induces major changes in the abundance of proteins associated with mitochondrial respiration. This study uncovers a large number of previously undescribed proteins potentially involved in the immune response.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_06:33:16.097.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Romain Hamelin
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-16 02:09:01	ID requested
⏵ 1	2025-05-07 06:33:16	announced

Publication List

10.1080/19336934.2025.2485685;
Rommelaere S, Sch, ü, pfer F, Armand F, Hamelin R, Lemaitre B, haemolymph after bacterial infection. Fly (Austin), 19(1):2485685(2025) [pubmed]

10.1080/19336934.2025.2485685;

Rommelaere S, Sch, ü, pfer F, Armand F, Hamelin R, Lemaitre B, haemolymph after bacterial infection. Fly (Austin), 19(1):2485685(2025) [pubmed]

Keyword List

submitter keyword: serine proteases, hemolymph, mass spectrometry, immunity, mitochondria,Drosophila melanogaster, antimicrobial peptides, Toll pathway, LC-MS/MS, Imd pathway

submitter keyword: serine proteases, hemolymph, mass spectrometry, immunity, mitochondria,Drosophila melanogaster, antimicrobial peptides, Toll pathway, LC-MS/MS, Imd pathway

Contact List

Bruno Lemaitre
contact affiliation	Global Health Institute, School of Life Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
contact email	bruno.lemaitre@epfl.ch
lab head
Romain Hamelin
contact affiliation	PCF
contact email	romain.hamelin@epfl.ch
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058897
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058897

PRIDE project URI

Repository Record List

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