PXD058880 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SAPrIm 2.0: A semi-automated protocol for mid-throughput soluble HLA immunopeptidomics |
Description | Human leukocyte antigen (HLA) molecules are pivotal in guiding human adaptive immune responses through their presentation of peptide ligands, collectively known as the immunopeptidome. This process is central to the development of cancer immunotherapies, such as vaccines and T-cell therapies. Profiling the immunopeptidome from plasma and other biofluids has gained increasing traction, as it offers a minimally invasive approach for monitoring disease states and immune responses toward cancer therapy. Here we present the second iteration of SAPrIm, a refined immunopeptidomics tool optimized for soluble HLA analysis. It can process up to 12 samples per batch within a day. This workflow is positioned to advance the field of immunopeptidomics by enabling efficient plasma-based comparative analyses and mid-size cohort studies. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-26 |
AnnouncementXML | Submission_2025-05-25_16:31:30.237.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | ERWIN TANUWIDJAYA |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | cysteinylation (disulfide with free L-cysteine); acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-12-15 20:03:22 | ID requested | |
⏵ 1 | 2025-05-25 16:31:31 | announced | |
Publication List
10.3389/fimmu.2025.1546629; |
Tanuwidjaya E, Lim Kam Sian TCC, Steele JR, Goncalves G, Woodhouse IB, Chang J, Ooi JD, Schittenhelm RB, Faridi P, SAPrIm 2.0: a semi-automated protocol for mid-throughput soluble HLA immunopeptidomics. Front Immunol, 16():1546629(2025) [pubmed] |
Keyword List
submitter keyword: sHLA, HLA, mass spectrometry, MHC, SAPrIm, immunopeptidomics,soluble HLA, plasma immunopeptidomics |
Contact List
Pouya Faridi |
contact affiliation | Department of Medicine, School of Clinical Science, Faculty of Medicine, Nursing & Health Science, Monash University, Clayton, VIC, Australia |
contact email | pouya.faridi@monash.edu |
lab head | |
ERWIN TANUWIDJAYA |
contact affiliation | Monash University |
contact email | Erwin.Tanuwidjaya1@monash.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058880
- Label: PRIDE project
- Name: SAPrIm 2.0: A semi-automated protocol for mid-throughput soluble HLA immunopeptidomics