PXD058880

PXD058880 is an original dataset announced via ProteomeXchange.

Title	SAPrIm 2.0: A semi-automated protocol for mid-throughput soluble HLA immunopeptidomics
Description	Human leukocyte antigen (HLA) molecules are pivotal in guiding human adaptive immune responses through their presentation of peptide ligands, collectively known as the immunopeptidome. This process is central to the development of cancer immunotherapies, such as vaccines and T-cell therapies. Profiling the immunopeptidome from plasma and other biofluids has gained increasing traction, as it offers a minimally invasive approach for monitoring disease states and immune responses toward cancer therapy. Here we present the second iteration of SAPrIm, a refined immunopeptidomics tool optimized for soluble HLA analysis. It can process up to 12 samples per batch within a day. This workflow is positioned to advance the field of immunopeptidomics by enabling efficient plasma-based comparative analyses and mid-size cohort studies.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_16:31:30.237.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ERWIN TANUWIDJAYA
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	cysteinylation (disulfide with free L-cysteine); acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-15 20:03:22	ID requested
⏵ 1	2025-05-25 16:31:31	announced

10.3389/fimmu.2025.1546629;

Tanuwidjaya E, Lim Kam Sian TCC, Steele JR, Goncalves G, Woodhouse IB, Chang J, Ooi JD, Schittenhelm RB, Faridi P, SAPrIm 2.0: a semi-automated protocol for mid-throughput soluble HLA immunopeptidomics. Front Immunol, 16():1546629(2025) [pubmed]

submitter keyword: sHLA, HLA, mass spectrometry, MHC, SAPrIm, immunopeptidomics,soluble HLA, plasma immunopeptidomics

Pouya Faridi
contact affiliation	Department of Medicine, School of Clinical Science, Faculty of Medicine, Nursing & Health Science, Monash University, Clayton, VIC, Australia
contact email	pouya.faridi@monash.edu
lab head
ERWIN TANUWIDJAYA
contact affiliation	Monash University
contact email	Erwin.Tanuwidjaya1@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058880

PRIDE project URI

• PRIDE
  1. PXD058880
     1. Label: PRIDE project
     2. Name: SAPrIm 2.0: A semi-automated protocol for mid-throughput soluble HLA immunopeptidomics