PXD058874

PXD058874 is an original dataset announced via ProteomeXchange.

Title	Identification of Amblyomma americanum antigens after vaccination with tick extracellular vesicles in white-tailed deer
Description	Anti-tick vaccines represent a promising alternative to chemical acaricides for the management of ticks on wildlife; however little progress has been made to produce an effective vaccine in hosts relevant to tick reproduction. Further, most of the antigens described are expressed during feeding in domesticated hosts and may not activate the immune system in wild hosts. We recently tested Amblyomma americanum salivary (SG) and midgut (MG) extracellular vesicles (EVs) as vaccine candidates in white-tailed deer (Odocoileus virginianus; WTD), which resulted in on-host female tick mortality. Herein, we describe our efforts to identify the antigenic proteins found within these vesicles. Within both SG- and MG-EVs, an enriched “core- cargo” was identified within both SG- and MG-EVs, including chaperones, small GTPases, and other proteins previously reported in small EVs was discovered. Label-free quantitative proteomics showed significant differences in protein cargo between MG and SG-EVs (333 proteins out of 516). Serum from three vaccinated WTD (pre-vaccinated and day 57 post vaccination) and one control WTD was used to immunoprecipitated antigenic proteins from SG- and MG-EV preparations. Proteomic analysis of immunoprecipitated proteins identified seven antigenic proteins in SG-EVs and one in MG-EVs that were statistically significantly different from the pre-vaccinated serum. Moreover, these proteins were unique to vaccinated animals (not precipitated by control serums). Additionally, two MG-EVs and 24 SG-EV proteins show antigenic potential. These proteins represent promising candidates for anti-tick vaccine design in WTD and other wildlife.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_16:45:42.713.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brian Mooney
SpeciesList	scientific name: Amblyomma americanum; NCBI TaxID: 6943; scientific name: Odocoileus virginianus; NCBI TaxID: 9874;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-14 13:38:30	ID requested
⏵ 1	2025-05-25 16:45:43	announced

10.3390/vaccines13040355;

Oliva Ch, á, vez A, Gonzalez J, Harvey C, Ribeiro-Silva CS, Leal-Galvan B, Persinger KA, Durski S, Olafson PU, Johnson TL, Antigens After Vaccination with Tick Extracellular Vesicles in White-Tailed Deer. Vaccines (Basel), 13(4):(2025) [pubmed]

submitter keyword: antigens,tick, vaccination

Adela Oliva Chávez
contact affiliation	Entomology Dept., 740 Russell Labss, University of Wisconsin-Madison, USA
contact email	olivachvez@wisc.edu
lab head
Brian Mooney
contact affiliation	University of Missouri, Columbia
contact email	mooneyb@missouri.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058874
     1. Label: PRIDE project
     2. Name: Identification of Amblyomma americanum antigens after vaccination with tick extracellular vesicles in white-tailed deer