PXD058865

PXD058865 is an original dataset announced via ProteomeXchange.

Title	Mitochondria-derived ROS regulation of tardigrade osmobiosis revealed by proteomics of Hypsibius exemplaris
Description	Tardigrades are microscopic invertebrates renowned for their ability to survive extreme environmental stress such as radiation, extreme temperatures, and desiccation. Yet, the biochemical mechanisms they utilize to survive these extremes are poorly understood. Herein, we implement proteomics to investigate the biomolecular underpinnings of tardigrade osmobiosis – a survival state in response to osmotic pressure. Using two solutes, the non-ionic sucrose and the ionic NaCl, we reveal that de novo gene expression is not required for osmobiosis induction. While sucrose and NaCl induce slightly different proteomic effects, both solutes lead to an increased abundance or oxidation of proteins involved in ER or mitochondrial activity. Further, we investigate the role of mitochondria in tardigrade osmobiosis and demonstrate that inhibition of the alternative oxidase (AOX) within the mitochondrial respiratory chain (MRC) increases the rate of osmobiosis formation across both sucrose and NaCl. Subsequent electron paramagnetic resonance (EPR) spectroscopy reveals an increased rate of reactive oxygen species (ROS) formation in osmobiotes with AOX inhibited, suggesting a regulation of osmobiosis through MRC-derived ROS. In sum, this work suggests mitochondrial-ROS signaling is necessary for tardigrade osmobiosis and further clarifies the biochemical mechanisms contributing to tardigrade extremotolerance.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-14_09:35:25.362.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058865
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Leslie Hicks
SpeciesList	scientific name: Hypsibius exemplaris; NCBI TaxID: 2072580;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	ZenoTOF 7600; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-13 14:19:25	ID requested
⏵ 1	2025-07-14 09:35:25	announced

10.6019/PXD058865;

10.1021/ACS.JPROTEOME.5C00222;

submitter keyword: proteomics,Tardigrades, reactive oxygen species, mitochondria, cysteine oxidation

Dr. Leslie Hicks
contact affiliation	Department of Chemistry, University of North Carolina at Chapel Hill
contact email	lmhicks@unc.edu
lab head
Leslie Hicks
contact affiliation	University of North Carolina at Chapel Hill
contact email	lmhicks@unc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058865
     1. Label: PRIDE project
     2. Name: Mitochondria-derived ROS regulation of tardigrade osmobiosis revealed by proteomics of Hypsibius exemplaris