PXD058858

PXD058858 is an original dataset announced via ProteomeXchange.

Title	Serine ADP-ribosylation on histones and PARP1 is a cellular target for ester-linked ubiquitylation
Description	ADP-ribosylation and ubiquitylation are key regulators of a wide variety of cellular processes, with the sophistication of their interplay becoming increasingly prominent, as illustrated by ADP-ribosylation-dependent ubiquitylation mediated by Legionella effectors. Recent biochemical studies have reported ester-linked ubiquitylation of ADP-ribose by DELTEX ligases, yet the modification sites of this dual-modification on cellular targets remain completely unknown. Here, our search for interactors of RNF114 revealed DNA damage-induced, PARP1/HPF1-dependent mono-ADPr on serine as a cellular target for ester-linked ubiquitylation. By developing a multifaceted proteomics strategy tailored to the chemical features of the composite modification and based on specific chemical disruption of its interaction with Di19-UIM, we identified unique ADP-ribosyl-linked serine ubiquitylation sites in human cells, including on histones and PARP1. We establish ADP-ribosyl-ubiquitylation as a cellular post-translational modification and propose that our tailored proteomic approach will reveal its widespread nature, along with additional conjugation chemistries, across diverse signaling pathways.
HostingRepository	PRIDE
AnnounceDate	2025-06-11
AnnouncementXML	Submission_2025-06-10_23:57:37.033.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilian Atanassov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-13 08:48:24	ID requested
⏵ 1	2025-06-10 23:57:37	announced

Dataset with its publication pending

submitter keyword: ADPr,DIA, DDA

Ivan Matic
contact affiliation	Max Planck Institute for Biology of Ageing Joseph-Stelzmann-Str. 9b 50931 Cologne Germany
contact email	imatic@age.mpg.de
lab head
Ilian Atanassov
contact affiliation	Max Planck Institute for Biology of Aging
contact email	Ilian.Atanassov@age.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058858
     1. Label: PRIDE project
     2. Name: Serine ADP-ribosylation on histones and PARP1 is a cellular target for ester-linked ubiquitylation