PXD058852

PXD058852 is an original dataset announced via ProteomeXchange.

Title	Temporal phosphoproteomics reveals circuitry of phased propagation in insulin signaling
Description	Insulin is a pleiotropic hormone that elicits its metabolic and mitogenic actions through numerous rapid and reversible protein phosphorylations. The temporal regulation of insulin’s intracellular signaling cascade is highly complex and insufficiently understood. We conducted a time-resolved analysis of the global insulin-regulated phosphoproteome of differentiated human primary myotubes derived from satellite cells of healthy donors using high-resolution mass spectrometry. Identification and tracking of ~13,000 phosphosites over time revealed a highly complex and coordinated network of transient phosphorylation and dephosphorylation events that can be allocated to time-phased regulation of distinct and non-overlapping subcellular pathways. Advanced network analysis combining protein-protein-interaction (PPI) resources and investigation of donor variability in phosphosite occupancy over time identifies novel putative candidates in non-canonical insulin signaling and key regulatory nodes that are likely essential for signal propagation. Lastly we found that insulin-regulated phosphorylation of the pre-catalytic spliceosome complex is associated with acute alternative splicing events in the transcriptome of human skeletal muscle. Our findings highlight the temporal relevance of protein phosphorylations and suggest that synchronized contributions of multiple signaling pathways form part of the circuitry for propagating information to insulin effector sites. In this sub-experiment of our main project, the global phosphoproteome of human primary myotubes under starving conditions over time in the absence of insulin has been investigated.
HostingRepository	PRIDE
AnnounceDate	2025-02-13
AnnouncementXML	Submission_2025-02-13_09:55:52.098.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Turewicz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-13 05:54:02	ID requested
⏵ 1	2025-02-13 09:55:52	announced

10.1038/S41467-025-56335-6;

submitter keyword: Phosphoproteomics, Donor variability, Insulin signaling,Skeletal muscle, mRNA splicing

Hadi Al-Hasani
contact affiliation	Institute for Clinical Biochemistry and Pathobiochemistry, German Diabetes Center (DDZ), Leibniz Center for Diabetes Research at Heinrich-Heine-University Düsseldorf, Auf´m Hennekamp 65, 40225 Düsseldorf, Germany
contact email	Hadi.Al-Hasani@ddz.de
lab head
Michael Turewicz
contact affiliation	Institute for Clinical Biochemistry and Pathobiochemistry German Diabetes Center (DDZ) Leibniz Center for Diabetes Research at Heinrich-Heine-University Düsseldorf Auf´m Hennekamp 65 40225 Düsseldorf Germany
contact email	michael.turewicz@ddz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD058852

PRIDE project URI

• PRIDE
  1. PXD058852
     1. Label: PRIDE project
     2. Name: Temporal phosphoproteomics reveals circuitry of phased propagation in insulin signaling