PXD058843

PXD058843 is an original dataset announced via ProteomeXchange.

Title	Light and polyphosphate kinase 2 cooperatively regulate the production of zero-valent sulfur in a deep-sea bacterium.
Description	The existence of light in various deep-sea environments has been well established. Our previous research found blue light promotes zero-valent sulfur (ZVS) production in Erythrobacter flavus 21-3, a bacterium isolated from the deep-sea cold seep. E. flavus 21-3 can convert thiosulfate to ZVS through a novel thiosulfate oxidation pathway comprising a thiosulfate dehydrogenase (TsdA) and two thiosulfohydrolases (SoxB). Using proteomic analysis, bacterial two-hybrid system and heterologous expression assays, we found that infrared light also promotes zero-valent sulfur (ZVS) production in E. flavus 21-3. The bacteriophytochrome (bphp) Ef2bphp-15570 autophosphorylated and activated GGDEF domain-containing protein D0Y83_RS00450 to produce c-di-GMP. Subsequently, the PilZ protein mPilZ-1753 bound to c-di-GMP and activated downstream sulfur oxidation pathways. During this process, polyphosphate kinase 2 (PPK2) affects the content of c-di-GMP by competing for GTP, thereby together c-di-GMP regulating ZVS production, as well as other metabolic processes in E. flavus 21-3. This study provides a novel insight into a deep-sea non-photosynthetic bacterium which sensing infrared light to regulate sulfur metabolism through a bacteriophytochrome photoreceptor, thus offering new understandings perspectives on microbial utilization of light energy.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_18:52:07.040.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhang tianhang
SpeciesList	scientific name: Erythrobacter; NCBI TaxID: 1041;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-13 01:15:16	ID requested
⏵ 1	2025-05-06 18:52:07	announced

10.1128/MSYSTEMS.00473-25;

submitter keyword: c-di-GMP,infrared light, polyphosphate kinase, deep-sea bacterium, zero-valent sulfur, blue light

Chaomin Sun
contact affiliation	The Institute of Oceanology Chinese Academy of Sciences
contact email	17853538258@163.com
lab head
zhang tianhang
contact affiliation	The Institute of Oceanology, Chinese Academy of Sciences
contact email	zhangtianhang@qdio.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058843
     1. Label: PRIDE project
     2. Name: Light and polyphosphate kinase 2 cooperatively regulate the production of zero-valent sulfur in a deep-sea bacterium.