PXD058811

PXD058811 is an original dataset announced via ProteomeXchange.

Title	BAHCC1 promotes gene expression in neuronal cells by antagonizing SIN3A-HDAC1
Description	Chromatin modifications play a key role in regulating gene expression during development and adult physiology. Histone acetylation, particularly H3K27ac, is associated with increased activity of gene regulatory elements such as enhancers and promoters. However, the regulation of the machinery that write, read, and erase this modification remains poorly understood. In particular, the SIN3A-HDAC1 complex possesses histone deacetylase activity, yet it commonly resides at active regulatory regions. Here, we study BAHCC1, a large chromatin-associated protein essential for viability, and recently reported to play a largely repressive role. We show that in neuronal lineage cells, BAHCC1 is mainly associated with regulatory elements marked with H3K27ac. BAHCC1 interacts and co-occupies shared genomic regions with the SIN3A scaffold protein, and its perturbations lead to altered acetylation and expression of proximal genes in a neuronal cell line and primary cortical neurons. The regulated genes are enriched for those functioning in neurogenesis and cell migration, and primary cortical neurons with reduced Bahcc1 expression display enhanced neurite outgrowth. We thus propose a model in which BAHCC1 antagonizes SIN3A histone deacetylation and positively regulates the expression of genes that are important for growth and migration-related processes in the neuronal lineage.
HostingRepository	PRIDE
AnnounceDate	2025-06-12
AnnouncementXML	Submission_2025-06-12_06:56:42.234.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alan Monziani
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	ACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-12 05:39:30	ID requested
⏵ 1	2025-06-12 06:56:42	announced

Dataset with its publication pending

submitter keyword: neurons, acetylation, SIN3A, HDAC,BAHCC1

Igor Ulitsky
contact affiliation	Department of Immunology and Regenerative Biology and Department of Molecular Neuroscience, Weizmann Institute of Science, Rehovot 76100, Israel
contact email	igor.ulitsky@weizmann.ac.il
lab head
Alan Monziani
contact affiliation	Weizmann Institute of Science
contact email	alan.monziani@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD058811

PRIDE project URI

• PRIDE
  1. PXD058811
     1. Label: PRIDE project
     2. Name: BAHCC1 promotes gene expression in neuronal cells by antagonizing SIN3A-HDAC1