PXD058769

PXD058769 is an original dataset announced via ProteomeXchange.

Title	Structurally Exclusive Teneurin Complexes Orchestrate Divergent Programs in Early Cortical Development
Description	Cortical migration is a complex process in which neurons migrate along radial glial cells (RGC) to form functional layers. Teneurins (Ten1-4) play a role by interacting with Latrophilins (Lphn/ADGRL1-3). Teneurins are also known as cell adhesion molecules, but how homophilic and heterophilic Teneurin interactions are integrated is unknown. Here, single-particle-cryo-EM data of Ten2 shows that canonical Latrophilin-binding is sterically incompatible with Ten2-dimerisation, making these interactions exclusive. We engineered surface mutations that specifically disrupt Ten2-Ten2 or Ten2-Latrophilin interactions. These are transferrable to Ten4, suggesting conserved binding mechanisms. Proteomics, in-vivo-gene-editing and super-resolution-microscopy show that Ten4 is expressed along RGC fibres and that migrating neurons switch from low-to-high Ten4-expression. Ten4 expression is highest in the cortical plate where Ten4-Ten4 interactions reduce RGC-attachment. In the intermediate zone, Ten4-Latrophilin interactions are required to promote neuron-RGC association. The results show how Ten4 orchestrates different stages of cortical migration by using a structural/functional switch between high-affinity Lphn interactions and low-affinity homophilic interactions, underpinning the integration of distinct migration programmes.
HostingRepository	PRIDE
AnnounceDate	2026-02-25
AnnouncementXML	Submission_2026-02-25_13:41:48.897.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vaishnavi Ravikumar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	complex glycosylation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-11 04:38:17	ID requested
⏵ 1	2026-02-25 13:41:49	announced

10.1101/2025.09.09.671438;

submitter keyword: None

Elena Seiradake
contact affiliation	Department of Biochemistry, University of Oxford, Oxford, UK
contact email	elena.seiradake@bioch.ox.ac.uk
lab head
Vaishnavi Ravikumar
contact affiliation	Dept. of Biochemistry, University of Oxford
contact email	vaishnavi.ravikumar@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD058769

PRIDE project URI

• PRIDE
  1. PXD058769
     1. Label: PRIDE project
     2. Name: Structurally Exclusive Teneurin Complexes Orchestrate Divergent Programs in Early Cortical Development