PXD058761

PXD058761 is an original dataset announced via ProteomeXchange.

Title	QKI dysregulation induces extensive splicing changes in T-cell acute lymphoblastic leukemia
Description	Understanding the molecular mechanisms underlying T-cell acute lymphoblastic leukemia (T-ALL) is essential for developing more effective therapeutic strategies. Despite advancements in T-ALL treatment, the role of RNA-binding proteins in the disease pathogenesis remains poorly understood. Here, we investigate the RNA-binding protein Quaking (QKI) in T-ALL, revealing it as a tumor suppressor with a critical role in splicing regulation. Through the analysis of two independent pediatric T-ALL cohorts, we demonstrate that QKI expression is frequently reduced in T-ALL, particularly within the HOXA subtype, and this reduction correlates with poor overall and event-free survival. Using T-ALL cell lines, we show that QKI depletion induces widespread splicing alterations, with numerous events corroborated in patient samples. Transcriptome profiling indicates that QKI downregulation leads to broad changes in gene expression, notably affecting pathways related to cell cycle progression, mitosis, and epithelial-mesenchymal transition. Functional assays demonstrate that QKI overexpression in T-ALL cells significantly reduces cell proliferation, induces G0/G1 cell cycle arrest, and limits leukemia progression and dissemination, ultimately improving survival in xenograft models. Together, these findings provide compelling evidence for QKI functions as a tumor suppressor and a key regulator of splicing in T-ALL.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:43:09.714.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Igor Fijalkowski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-11 01:18:13	ID requested
⏵ 1	2026-06-07 16:43:10	announced

10.3324/haematol.2025.287809;

Palhais B, Sharma ND, Fijalkowski I, Pieters T, Deforce D, Nieuwerburgh FV, Mestdagh P, Ntziachristos P, Matlawska-Wasowska K, Vlierberghe PV, dysregulation induces extensive splicing changes in T-cell acute lymphoblastic leukemia. Haematologica, 111(6):1953-1968(2026) [pubmed]

submitter keyword: Human, Splicing, T-ALL

Panagiotis Ntziachristos
contact affiliation	Center for Medical Genetics, Ghent University and University Hospital, Ghent, Belgium
contact email	panagiotis.ntziachristos@ugent.be
lab head
Igor Fijalkowski
contact affiliation	Ugent
contact email	igor.fijalkowski@ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD058761

PRIDE project URI

• PRIDE
  1. PXD058761
     1. Label: PRIDE project
     2. Name: QKI dysregulation induces extensive splicing changes in T-cell acute lymphoblastic leukemia