PXD058735

PXD058735 is an original dataset announced via ProteomeXchange.

Title	Species disparity and sensitivity differences in opsonization and phagocyte recognition of poly-2-alkyl-2-oxazoline-coated nanoparticles
Description	Poly(ethylene glycol) (PEG) is widely used in engineering of clinical nanomedicines; however, the emerging PEG immunogenicity is compromising their therapeutic efficacy. Polyoxazolines are gaining popularity as PEG alternatives, since “polyoxazolination” confers stealthing behaviour to nanomedicines in the preclinical mouse model. Here, using nanoparticles coated with either poly-2-methyl-2-oxazoline (PMOXA) or poly-2-ethyl-2-oxazoline (PEOXA), we show species differences in nanoparticle opsonization and phagocytic cell responses. PMOXA- and PEOXA-coated nanoparticles evade murine serum opsonization and phagocytic clearance. Polyoxazolinated nanoparticles, however, undergo differential opsonization in porcine and human sera, resulting in nanoparticle recognition by different phagocytic cells differently. We identify ficolin 2 (FCN2) as a monocyte-specific opsonin in pigs, whereas in human sera, FCN opsonization is isoform-dependent with inter-individual variability. Polymer recognition is through the S2 domain of FCN2 which sense hydrogen bond forming chemical moieties and hydrophobic patterns of polyoxazolines. Contrary to the porcine model, both FCN2 and complement promote nanoparticle uptake by human monocytes. On the other hand, nanoparticle uptake by human and porcine macrophage is complement-dependent. These findings highlight the importance of species differences in innate immune recognition of nanomaterials’ molecular patterns, and are relevant to the selection and chemical design of polymers for engineering of the next generation of stealth nanoparticles.
HostingRepository	PRIDE
AnnounceDate	2025-03-11
AnnouncementXML	Submission_2025-03-11_00:40:22.474.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giorgio Arrigoni
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-10 08:44:02	ID requested
⏵ 1	2025-03-11 00:40:22	announced

10.1038/S41467-025-57648-2;

submitter keyword: Opsonins

Complement

Ficolins

Nanoparticles

Polyoxazolines

Pattern Recognition Molecules

Phagocytes

Preclinical modelsOpsonins

Complement

Ficolins

Nanoparticles

Polyoxazolines

Pattern Recognition Molecules

Phagocytes

Preclinical models

Giorgio Arrigoni
contact affiliation	Department of Biomedical Sciences, University of Padova, Italy
contact email	giorgio.arrigoni74@gmail.com
lab head
Giorgio Arrigoni
contact affiliation	University of Padova
contact email	giorgio.arrigoni74@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD058735

PRIDE project URI

• PRIDE
  1. PXD058735
     1. Label: PRIDE project
     2. Name: Species disparity and sensitivity differences in opsonization and phagocyte recognition of poly-2-alkyl-2-oxazoline-coated nanoparticles