PXD058727

PXD058727 is an original dataset announced via ProteomeXchange.

Title	Cavβ2e translocates from the plasma membrane to the nucleus and potentially regulates gene expression
Description	Voltage-gated calcium channels (VGCCs) play a crucial role in various cellular processes. These channels consist of different subunits, including the Cavβ subunit. In this study, our focus was to investigate the molecular mechanisms underlying the nuclear trafficking of a specific Cavβ2 splice variant, Cavβ2e, and its potential role in regulating gene expression. Previous reports have demonstrated that electrostatic interactions between positively charged amino acids in the N-terminus of Cavβ2e and negatively charged phospholipids of the plasma membrane facilitate its anchoring to the membrane. Our findings reveal that Cavβ2e can translocate from the plasma membrane to the nucleus upon activation of PLC. Further analysis identified specific amino acids within the N-terminus of Cavβ2e that are essential for its nuclear targeting, acting as a nuclear localization signal. Mutations in these amino acids resulted in reduced nuclear localization, supporting their involvement in the nuclear translocation of Cavβ2e. We also investigated the presence of a nuclear export signal within Cavβ2e and identified a crucial dileucine motif that is involved in its trafficking between the nucleus and cytosol. Moreover, we studied the function of nuclear Cavβ2e and found that it potentially can regulate gene expression. In conclusion, our study provides insights into the potential regulation of gene expression in native systems through the translocation of Cavβ2e from the plasma membrane to the nucleus upon activation of PLC.
HostingRepository	PRIDE
AnnounceDate	2025-04-04
AnnouncementXML	Submission_2025-04-04_08:08:54.439.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058727
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Svitlana Rozanova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-10 03:05:00	ID requested
⏵ 1	2025-04-04 08:08:54	announced

10.6019/PXD058727;

10.3389/FPHYS.2025.1555934;

submitter keyword: nuclear localization signal,voltage-gated calcium channels, Cavβ, PLC, cellular signaling, nuclear translocation

Katrin Marcus-Alic
contact affiliation	Director of the Institute Medizinisches Proteom-Center Gesundheitscampus 4 D-44801 Bochum Germnany
contact email	katrin.marcus@ruhr-uni-bochum.de
lab head
Svitlana Rozanova
contact affiliation	Ruhr University Bochum, Medical Faculty, Medizinisches Proteom-Center
contact email	Svitlana.Rozanova@ruhr-uni-bochum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058727
     1. Label: PRIDE project
     2. Name: Cavβ2e translocates from the plasma membrane to the nucleus and potentially regulates gene expression