PXD058725
PXD058725 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Embedding a ribonuclease in the spore crust couples gene expression to spore development in Bacillus subtilis |
| Description | Faced with nutritional stress, some bacteria form endospores that can survive extreme conditions for long periods of time; yet the function of many proteins expressed during sporulation remains a mystery. We show that one such protein, KapD, is a 3 prime exoribonuclease expressed under control of the mother cell-specific transcription factors SigE and SigK in B. subtilis. KapD assembles dynamically over the spore surface through a direct interaction with the major crust protein CotY. KapD catalytic activity is required for the normal adhesiveness of spore surface layers. We identify the sigK mRNA as a key substrate for KapD and show that its sequestration by CotY controls the stability of the sigK transcript. SigK is controlled through excision of a prophage-like element, transcriptional regulation, and removal of an inhibitory pro-sequence. We reveal a fourth, posttranscriptional layer of control of sigK expression that couples late-stage gene expression in the mother cell to spore morphogenesis. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-12-17 |
| AnnouncementXML | Submission_2024-12-17_06:29:53.286.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hamon |
| SpeciesList | scientific name: Bacillus subtilis; NCBI TaxID: 1423; |
| ModificationList | L-methionine sulfoxide; Acetyl; Carbamidomethyl |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-12-10 01:21:17 | ID requested | |
| ⏵ 1 | 2024-12-17 06:29:53 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Post-transcriptional regulation, Cell development, Sporulation, RNA degradation, Q-Exactive plus, Maxquant, DatasetType:Proteomics |
Contact List
| Alexandre D'Halluin | |
|---|---|
| contact affiliation | Institut de Biologie Physico-Chimique |
| contact email | alexandre.dhalluin@ibpc.fr |
| lab head | |
| Hamon | |
| contact affiliation | IBPC-CNRS |
| contact email | marion.hamon@ibpc.fr |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000096627/ |
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