PXD058681

PXD058681 is an original dataset announced via ProteomeXchange.

Title	Cancer-associated mutations impair the capability of CRM1 to regulate NPAT condensation and histone locus body
Description	Importins could inhibit the condensation of RNA-binding proteins, while it is proposed that exportins could not elicit a similar function. Here, we identified exportin CRM1 binds nuclear protein NPAT, which initiates and maintains the formation of histone locus body (HLB), a membrane-less nuclear body determining histone transcription. CRM1 drives the nuclear export of NPAT by targeting a cryptical nuclear export signal (NES) motif within the LisH domain. The LisH domain contributes to NPAT condensation by mediating its self-association. Mechanistically, CRM1 competitively occupies the self-association sites in the NES motif to suppress NPAT condensation. In contrast, the two recurrent CRM1 E571K and E571G mutants could not regulate NPAT condensation and HLB remodeling due to their impaired binding to the NES of NPAT. Based on the “competitive occupation” model, we designed a LisH domain-derived short peptide that competes with homotypic intermolecular interactions of NPAT to perturb HLB formation. Our findings reveal that exportin regulates nuclear protein condensation via a “competitive occupation” strategy.
HostingRepository	iProX
AnnounceDate	2024-12-08
AnnouncementXML	Submission_2024-12-08_20:06:06.125.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuibo Xu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-08 20:05:16	ID requested
⏵ 1	2024-12-08 20:06:06	announced

Dataset with its publication pending

submitter keyword: condensation, phase separation, exportin, histone locus body, NPAT, replication-dependent histone

Yiting Zhou
contact affiliation	Zhejiang University
contact email	Zhouyt@zju.edu.cn
lab head
Shuibo Xu
contact affiliation	Zhejiang University
contact email	xushuibo@zju.edu.cn
dataset submitter

iProX dataset URI