PXD058677

PXD058677 is an original dataset announced via ProteomeXchange.

Title	Lable- Free protein mass spectrometry for ht-SKPs-EV and FBs-EV
Description	We apply 3D directed induced transfer culture system alongsideand ultra-high speed centrifugation technology to break through the mass production bottleneck of ht-SKPs-EV (human transformed skin-derived precursors). We hope to compare its protein expression with that of fibroblasts extracellular vesicles (FBs-EV) in traditional adherent culture mode.The experimental procedure for analyzing ht-SKPs-EV and FBs-EV proteins using proteomics involves extracting proteins from ht-SKPs-EV and FBs-EV, subjecting them to gel electrophoresis, transferring the proteins onto a membrane, probing with specific antibodies, and detecting the protein bands to assess expression levels.
HostingRepository	iProX
AnnounceDate	2024-12-06
AnnouncementXML	Submission_2024-12-08_19:50:32.101.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lingyun Zhao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-08 19:49:33	ID requested
⏵ 1	2024-12-08 19:50:32	announced

Dataset with its publication pending

submitter keyword: Protein mass spectrometry, extracellular vesicles, skin-derived precursors

Li Li
contact affiliation	West China Hospital, Sichuan University
contact email	lilihx_scu@scu.edu.cn
lab head
Lingyun Zhao
contact affiliation	West China Hospital, Sichuan University
contact email	zhaolingyun96@163.com
dataset submitter

iProX dataset URI