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PXD058676

PXD058676 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative Analysis of Lysine Malonylation Proteome Unravels Physiological and Biochemical Mechanism in Responding to Salicylic Acid in Roots of Platycodon grandiflorus
DescriptionSalicylic acid, as a plant hormone, significantly affects physiological and biochemical indexes of such as soluble sugar, malondialdehyde content, peroxidase and superoxide dismutase enzyme activity in Platycodon grandiflorus. Lysine malonylation is a post-translational modification that involves various cellular functions in plants, though it is rarely studied, especially in medicinal plants. This study aimed to perform a comparative quantitative proteomic study of malonylation modification on P. grandiflorus root proteins after salicylic acid treatment using Western Blot with specific antibodies, affinity enrichment and LC-MS/MS analysis methods. The analysis identified 1,907 malonyl sites for 809 proteins, with 414 proteins and 798 modification sites quantified with high confidence. Post-treatment, 361 proteins were up-regulated, and 310 were down-regulated. Bioinformatics analysis revealed that malonylation in P. grandiflorus is primarily involved in pho-tosynthesis and carbon metabolism. Physiological and biochemical analysis showed that saliylic acid treatment increased the malondialdehyde levels, soluble protein, superoxide dismutase, and peroxidase activity but did not significantly affect the total saponins content in P. grandiflorus. These findings provide an important basis for exploring the molecular mechanisms of P. grandi-florus following salicylic acid treatment and enhance understanding of the biological function of protein lysine malonylation in plants.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_05:34:10.505.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWanyue Ding
SpeciesList scientific name: Platycodon grandiflorus; NCBI TaxID: 94286;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro 2
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-12-08 19:49:09ID requested
12025-05-07 05:34:11announced
Publication List
10.3390/ijms26031392;
Ding W, Duan Y, Wang Y, Fan J, Rao W, Xing S, . Int J Mol Sci, 26(3):(2025) [pubmed]
Keyword List
submitter keyword: Platycodon grandiflorus
Salicylic acid
Lysine malonylation
Medicinal plants
Qualitative proteome
Post-translational modification.
Contact List
Wanyue Ding
contact affiliationCollege of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.
contact emaildwy1020304050@163.com
lab head
Wanyue Ding
contact affiliationPTM Biolabs Inc.
contact emaildwy1020304050@163.com
dataset submitter
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Dataset FTP location
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