PXD058676

PXD058676 is an original dataset announced via ProteomeXchange.

Title	Quantitative Analysis of Lysine Malonylation Proteome Unravels Physiological and Biochemical Mechanism in Responding to Salicylic Acid in Roots of Platycodon grandiflorus
Description	Salicylic acid, as a plant hormone, significantly affects physiological and biochemical indexes of such as soluble sugar, malondialdehyde content, peroxidase and superoxide dismutase enzyme activity in Platycodon grandiflorus. Lysine malonylation is a post-translational modification that involves various cellular functions in plants, though it is rarely studied, especially in medicinal plants. This study aimed to perform a comparative quantitative proteomic study of malonylation modification on P. grandiflorus root proteins after salicylic acid treatment using Western Blot with specific antibodies, affinity enrichment and LC-MS/MS analysis methods. The analysis identified 1,907 malonyl sites for 809 proteins, with 414 proteins and 798 modification sites quantified with high confidence. Post-treatment, 361 proteins were up-regulated, and 310 were down-regulated. Bioinformatics analysis revealed that malonylation in P. grandiflorus is primarily involved in pho-tosynthesis and carbon metabolism. Physiological and biochemical analysis showed that saliylic acid treatment increased the malondialdehyde levels, soluble protein, superoxide dismutase, and peroxidase activity but did not significantly affect the total saponins content in P. grandiflorus. These findings provide an important basis for exploring the molecular mechanisms of P. grandi-florus following salicylic acid treatment and enhance understanding of the biological function of protein lysine malonylation in plants.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_05:34:10.505.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wanyue Ding
SpeciesList	scientific name: Platycodon grandiflorus; NCBI TaxID: 94286;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-08 19:49:09	ID requested
⏵ 1	2025-05-07 05:34:11	announced

10.3390/ijms26031392;

Ding W, Duan Y, Wang Y, Fan J, Rao W, Xing S, . Int J Mol Sci, 26(3):(2025) [pubmed]

submitter keyword: Platycodon grandiflorus

Salicylic acid

Lysine malonylation

Medicinal plants

Qualitative proteome

Post-translational modification.

Wanyue Ding
contact affiliation	College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China.
contact email	dwy1020304050@163.com
lab head
Wanyue Ding
contact affiliation	PTM Biolabs Inc.
contact email	dwy1020304050@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058676
     1. Label: PRIDE project
     2. Name: Quantitative Analysis of Lysine Malonylation Proteome Unravels Physiological and Biochemical Mechanism in Responding to Salicylic Acid in Roots of Platycodon grandiflorus