PXD058676 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative Analysis of Lysine Malonylation Proteome Unravels Physiological and Biochemical Mechanism in Responding to Salicylic Acid in Roots of Platycodon grandiflorus |
Description | Salicylic acid, as a plant hormone, significantly affects physiological and biochemical indexes of such as soluble sugar, malondialdehyde content, peroxidase and superoxide dismutase enzyme activity in Platycodon grandiflorus. Lysine malonylation is a post-translational modification that involves various cellular functions in plants, though it is rarely studied, especially in medicinal plants. This study aimed to perform a comparative quantitative proteomic study of malonylation modification on P. grandiflorus root proteins after salicylic acid treatment using Western Blot with specific antibodies, affinity enrichment and LC-MS/MS analysis methods. The analysis identified 1,907 malonyl sites for 809 proteins, with 414 proteins and 798 modification sites quantified with high confidence. Post-treatment, 361 proteins were up-regulated, and 310 were down-regulated. Bioinformatics analysis revealed that malonylation in P. grandiflorus is primarily involved in pho-tosynthesis and carbon metabolism. Physiological and biochemical analysis showed that saliylic acid treatment increased the malondialdehyde levels, soluble protein, superoxide dismutase, and peroxidase activity but did not significantly affect the total saponins content in P. grandiflorus. These findings provide an important basis for exploring the molecular mechanisms of P. grandi-florus following salicylic acid treatment and enhance understanding of the biological function of protein lysine malonylation in plants. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_05:34:10.505.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wanyue Ding |
SpeciesList | scientific name: Platycodon grandiflorus; NCBI TaxID: 94286; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro 2 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-12-08 19:49:09 | ID requested | |
⏵ 1 | 2025-05-07 05:34:11 | announced | |
Publication List
Keyword List
submitter keyword: Platycodon grandiflorus |
Salicylic acid |
Lysine malonylation |
Medicinal plants |
Qualitative proteome |
Post-translational modification. |
Contact List
Wanyue Ding |
contact affiliation | College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China. |
contact email | dwy1020304050@163.com |
lab head | |
Wanyue Ding |
contact affiliation | PTM Biolabs Inc. |
contact email | dwy1020304050@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058676
- Label: PRIDE project
- Name: Quantitative Analysis of Lysine Malonylation Proteome Unravels Physiological and Biochemical Mechanism in Responding to Salicylic Acid in Roots of Platycodon grandiflorus