PXD058656

PXD058656 is an original dataset announced via ProteomeXchange.

Title	A BAG-1-inhibitory peptide, GO-Pep, suppresses c-Raf activity in cancer
Description	Herein, we explore the druggability of BAG-1S (the smallest isoform) with a specific emphasis on its interaction with C-RAF. We firstly characterize the higher order structure of BAG-1S structure to determine druggable sites by HDX-MS. We then map the BAG-1S:c-Raf interface – identifying a 20 amino acid region likely to interact with c-Raf. Following which, we use mutational analysis of BAG-1S to show specific amino acids that disrupt the BAG-1S:c-Raf interaction, downstream signaling and cell growth. Finally, we develop a BAG-1S interacting peptide (from c-Raf) that disrupts BAG-1 protein:protein interactions (including C/B-RAF, CHIP and HSP70) and modified with a cell-penetrating motif which inhibits the growth of cancer cells with associated signaling inhibition and induction of apoptosis.
HostingRepository	PRIDE
AnnounceDate	2024-12-17
AnnouncementXML	Submission_2024-12-17_00:18:33.772.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gizem Dinler Doganay
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-06 10:43:22	ID requested
⏵ 1	2024-12-17 00:18:34	announced

Dataset with its publication pending

submitter keyword: BAG-1, HDX-MS

Gizem Dinler Doganay
contact affiliation	Department of Molecular Biology and Genetics, Faculty of Arts and Science, Istanbul Technical University, Istanbul, Türkiye
contact email	gddoganay@itu.edu.tr
lab head
Gizem Dinler Doganay
contact affiliation	Department of Molecular Biology and Genetics, Faculty of Arts and Science, Istanbul Technical University, Istanbul
contact email	gddoganay@itu.edu.tr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD058656

PRIDE project URI

• PRIDE
  1. PXD058656
     1. Label: PRIDE project
     2. Name: A BAG-1-inhibitory peptide, GO-Pep, suppresses c-Raf activity in cancer