PXD058653

PXD058653 is an original dataset announced via ProteomeXchange.

Title	Integrated proteomic, DNA methylation, and targeted Next Generation Sequencing reveals relevant heterogeneity in lower-grade meningioma and a new target in NF2 mutated meningiomas
Description	Meningiomas, the most common primary brain tumours, are classified by the World Health Organization (WHO) into grades 1, 2, and 3. Some grade 1 tumours exhibit increased clinical aggressiveness with the biallelic mutation of Neurofibromatosis 2 (NF2) being the most frequent mutation reported. In our study, we analysed the most common driver mutations (NF2, AKT1, KLF4, and TRAF7) in meningioma by proteomics and genomics focussing on lower grade tumours. Our genomic data revealed co-occurrences of non-NF2 mutations in lower-grade meningiomas suggesting synergistic effects supporting tumour growth. NF2-/- meningiomas showed distinct proteomic clustering, with different mutations found in these clusters. Additionally, proteomics identified ANXA3 in NF2-/- meningioma and its role in proliferation was confirmed in grade1 and subsequently grade 3 tumours in vitro and with abolished growth when tested in meningioma mouse model. These findings highlight new targets in different meningioma backgrounds presenting ANXA3 as a potential therapeutic target for meningioma treatment.
HostingRepository	PRIDE
AnnounceDate	2025-07-02
AnnouncementXML	Submission_2025-07-02_03:18:32.462.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vikram Sharma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-06 08:24:47	ID requested
⏵ 1	2025-07-02 03:18:33	announced

10.1016/j.ebiom.2025.105814;

Shah M, Akther Y, Adams CL, Banton MC, Sharma V, Ercolano E, Hilton DA, Kurian KM, Laraba L, Woznica W, Parkinson DB, de Assis LJ, Hanemann CO, Integrated proteomic and targeted Next Generation Sequencing reveal relevant heterogeneity in lower-grade meningioma and ANXA3 as a new target in NF2 mutated meningiomas. EBioMedicine, ():105814(2025) [pubmed]

submitter keyword: Genomics, NF2, Proteomics,Meningioma

Vikram Sharma
contact affiliation	School of Biomedical Sciences, University of Plymouth, UK
contact email	vikram.sharma@plymouth.ac.uk
lab head
Vikram Sharma
contact affiliation	Plymouth University Peninsula Schools of Medicine and Dentistry
contact email	vikram.sharma@plymouth.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD058653

PRIDE project URI

• PRIDE
  1. PXD058653
     1. Label: PRIDE project
     2. Name: Integrated proteomic, DNA methylation, and targeted Next Generation Sequencing reveals relevant heterogeneity in lower-grade meningioma and a new target in NF2 mutated meningiomas