PXD058644

PXD058644 is an original dataset announced via ProteomeXchange.

Title	Structural and Functional Glycosylation of the Abdala COVID-19 Vaccine
Description	Abdala is a COVID-19 vaccine produced in Pichia pastoris and is based on the receptor-binding domain (RBD) of the SARS-CoV-2 spike. Abdala is currently approved for use in multiple countries with clinical trials confirming its safety and efficacy in preventing severe illness and death. Although P. pastoris is used as an expression system for protein-based vaccines, yeast glycosylation remains largely uncharacterised across immunogens. Here, we characterise N-glycan structures and their site of attachment on Abdala and show how yeast-specific glycosylation decreases binding to the ACE2 receptor and a receptor-binding motif (RBM) targeting antibody compared to the equivalent mammalian-derived RBD. Reduced receptor and antibody binding is attributed to changes in conformational dynamics resulting from N-glycosylation. These data highlight the critical importance of glycosylation in vaccine design and demonstrate how individual glycans can influence host interactions and immune recognition via protein structural dynamics.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_05:28:27.726.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sean Burnap
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-06 04:13:41	ID requested
⏵ 1	2025-05-07 05:28:28	announced

Burnap SA, Calvaresi V, Cabrera G, Pousa S, Limonta M, Ramos Y, Gonz, á, lez LJ, Harvey DJ, Struwe WB, Structural and Functional Glycosylation of the Abdala COVID-19 Vaccine. Glycobiology, 35(3):(2025) [pubmed]

10.1093/glycob/cwaf001;

submitter keyword: Glycosylation, COVID, SARS,Abdala

Weston Struwe
contact affiliation	Department of Biochemistry, Dorothy Crowfoot Hodgkin Building, University of Oxford, South Parks Road, OX1 3QU, UK.
contact email	weston.struwe@bioch.ox.ac.uk
lab head
Sean Burnap
contact affiliation	University of Oxford
contact email	sean.burnap@bioch.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058644

PRIDE project URI

• PRIDE
  1. PXD058644
     1. Label: PRIDE project
     2. Name: Structural and Functional Glycosylation of the Abdala COVID-19 Vaccine