PXD058617

PXD058617 is an original dataset announced via ProteomeXchange.

Title	A Class 1 OLD family nuclease encoded by Vibrio cholerae is countered by a vibriophage-encoded direct inhibitor
Description	Bacteria are constantly threatened by their viral predators (phages), which has resulted in the development of defense systems for bacterial survival. One family of defense systems found widely across bacteria are OLD (for overcoming lysogeny defect) family nucleases. Despite recent discoveries regarding Class 2 and 4 OLD family nucleases and how phages overcome them, Class 1 OLD family nucleases warrant further study as there has only been one anti-phage Class 1 OLD family nuclease described to date. Here, we identify the Vibrio cholerae-encoded Class 1 OLD family nuclease Vc OLD and describe its disruption of genome replication of the lytic vibriophage ICP1. Furthermore, we examine its in vitro activity, identifying Vc OLD as a DNA nickase. Finally, we identify the first direct inhibitor of a Class 1 OLD family nuclease, the ICP1-encoded Oad1. Our research further illuminates Class 1 OLD family nucleases’ role in phage defense and explores the dynamic arms race between V. cholerae and its predatory phage ICP1.
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-14_16:21:55.267.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058617
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kimberley Seed
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-05 09:12:57	ID requested
⏵ 1	2025-09-14 16:21:56	announced

10.6019/PXD058617;

10.1093/nar/gkaf728;

Patel KM, Seed KD, A DNA nicking Class 1 OLD family nuclease mediates phage defense in Vibrio cholerae and is countered by a phage-encoded inhibitor. Nucleic Acids Res, 53(16):(2025) [pubmed]

submitter keyword: phage, Vibrio cholerae,Nuclease

Kimberley Seed
contact affiliation	PI. Associate Professor Department of Plant and Microbial Biology. UCB.
contact email	kseed@berkeley.edu
lab head
Kimberley Seed
contact affiliation	University of California Berkeley
contact email	kseed@berkeley.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058617
     1. Label: PRIDE project
     2. Name: A Class 1 OLD family nuclease encoded by Vibrio cholerae is countered by a vibriophage-encoded direct inhibitor