PXD058604

PXD058604 is an original dataset announced via ProteomeXchange.

Title	Widespread telomere bacteriophages equip their hosts with potent inter-bacterial weapons
Description	Bacteriophage (phage) are viruses that can kill bacteria, but also mediate gene transfer for bacterial evolution. The telomere phages are a curious form using telomere-like structures to replicate their genomes as linear extrachromosomal elements. Here we find that telomere phages are widely distributed in bacteria, being highly prevalent in Klebsiella species. We established a model system to investigate telomere phage biology and find only a small set of phage proteins are expressed in phage-host cells, including a toxin – telocin - that kills other Klebsiella strains. We identify and validate other telocins in the genomes of other, widespread Klebsiella telomere phages. Thus, telomere phages are widespread elements encoding diverse antibacterial weapons and we discuss the prospect of using telocins for precision editing of microbial populations.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_05:41:50.591.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ralf Schittenhelm
SpeciesList	scientific name: Klebsiella variicola subsp. variicola; NCBI TaxID: 2590157;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-05 01:42:46	ID requested
⏵ 1	2025-05-07 05:41:51	announced

Byers SMH, Rocker A, Nguyen TNT, Rosas NC, Taiaroa G, Tan KS, Li Y, Wilksch JJ, Steele JR, Schittenhelm RB, Dunstan RA, Short FL, Lithgow T, Telomere bacteriophages are widespread and equip their bacterial hosts with potent interbacterial weapons. Sci Adv, 11(18):eadt1627(2025) [pubmed]

10.1126/sciadv.adt1627;

submitter keyword: telocin, Klebsiella, toxin-antitoxin, bacteriocin,phages, pore-forming, telomere phage

Trevor Lithgow
contact affiliation	Centre to Impact AMR, Monash University; Clayton, 3800, Australia Infection Program, Biomedicine Discovery Institute and Department of Microbiology, Monash University; Clayton, 3800, Australia
contact email	trevor.lithgow@monash.edu
lab head
Ralf Schittenhelm
contact affiliation	Monash University
contact email	ralf.schittenhelm@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058604

PRIDE project URI

• PRIDE
  1. PXD058604
     1. Label: PRIDE project
     2. Name: Widespread telomere bacteriophages equip their hosts with potent inter-bacterial weapons