PXD058574

PXD058574 is an original dataset announced via ProteomeXchange.

Title	Transcription factors form a ternary complex with NIPBL/MAU2 to localize cohesin at enhancers
Description	While the cohesin complex is a key player in genome architecture, how it localizes to specific chromatin sites is not well understood. Recently, we and others have proposed that direct interactions with transcription factors (TFs) lead to the localization of the cohesin-loader complex (NIPBL/MAU2) within enhancers. Here, we identify two clusters of protein-protein interaction (PPI) motifs within the NIPBL sequence that regulate NIPBL dynamics, interactome, and NIPBL-dependent transcriptional programs. These PPIMs directly interact with chromatin-associated proteins, including TFs and one of them is necessary for the maintenance of the NIPBL-MAU2 heterodimer. Finally, using the glucocorticoid receptor (GR) as a model, we investigate its interaction surfaces with NIPBL and MAU2 using AlphaFold2 and surface plasmon resonance to uncover a TF-NIPBL-MAU2 ternary complex and present its importance in GR-dependent gene regulation.
HostingRepository	PRIDE
AnnounceDate	2025-04-17
AnnouncementXML	Submission_2025-04-17_02:27:15.893.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058574
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Didier Vertommen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	O4'-phospho-L-tyrosine; O-phospho-L-threonine; monohydroxylated residue; O-phospho-L-serine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-04 08:19:09	ID requested
⏵ 1	2025-04-17 02:27:16	announced

10.6019/PXD058574;

submitter keyword: cohesin, NIPBL, transcription factors, interactome

Franck Dequiedt
contact affiliation	Laboratory of Gene Expression and Cancer, GIGA-Molecular & Computational Biology, University of Liège, 4000 Liège, Belgium
contact email	fdequiedt@uliege.be
lab head
Didier Vertommen
contact affiliation	UCL - de Duve Institute, Brussels Belgium
contact email	didier.vertommen@uclouvain.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD058574

PRIDE project URI

• PRIDE
  1. PXD058574
     1. Label: PRIDE project
     2. Name: Transcription factors form a ternary complex with NIPBL/MAU2 to localize cohesin at enhancers