PXD058529

PXD058529 is an original dataset announced via ProteomeXchange.

Title	Structure of human PINK1 at a mitochondrial TOM/VDAC array
Description	We report here the structure of human PINK1 stabilized mid-import at an array of endogenous TOM and VDAC complexes purified from human mitochondria. In our structure, two TOM core complexes are separated by a dimer of VDAC2 channels, and an inactive PINK1 dimer is inserted into the VDAC-proximal TOM40 pores, positioning the dimerized PINK1 kinase domains directly atop the VDAC channels. The structure explains how PINK1 interacts with subunits of the TOM complex and rationalizes several known EOPD patient mutations. The unexpected arrangement of TOM with VDAC suggests a structural role for VDAC channels in forming mitochondrial import arrays, and the stalled PINK1 import intermediate illuminates how a presequence import substrate is guided across the mitochondrial outer membrane.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-05_16:04:31.218.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Dagley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-03 06:06:51	ID requested
⏵ 1	2025-05-05 16:04:31	announced

10.1126/SCIENCE.ADU6445;

submitter keyword: PINK1, CryoEM, XL-MS, Parkinson's diseease

Prof. David Komander
contact affiliation	The Walter and Eliza Hall Institute of Medical Research Professor of Medical Biology, University of Melbourne, Australia
contact email	dk@wehi.edu.au
lab head
Laura Dagley
contact affiliation	WEHI
contact email	dagley.l@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058529

PRIDE project URI

• PRIDE
  1. PXD058529
     1. Label: PRIDE project
     2. Name: Structure of human PINK1 at a mitochondrial TOM/VDAC array