PXD058528

PXD058528 is an original dataset announced via ProteomeXchange.

Title	Computational prediction combined with proteogenomics unveils widespread non-AUG translation initiation events in plants
Description	Non-AUG translation initiation is crucial for regulating translation, which can generate N-terminally extended protein isoforms with diverse biological functions. However, a comprehensive view of non-AUG translation initiation events with N-terminal extensions (NTEs) across monocots and dicots remains limited. Here, we identified a total of 879 transcripts undergoing non-AUG translation initiation events with NTEs in maize, rice and soybean using a computational prediction-combined proteogenomic strategy. Over half the non-AUG initiated candidates had multiple putative upstream translation initiation sites (uTISs), with GUG being the most common uTIS, followed by CUG in monocots and AUU in the dicot. Sequence context analyses showed that these putative uTISs tended to have Kozak sequence and high AG content favoring initiation. Additionally, non-AUG initiated candidates had longer 5' untranslated regions (UTRs) across all species, whereas other transcript features showed contrasting trends between monocots and the dicot. Furthermore, non-AUG initiated candidates in maize were implicated in essential cellular processes and diverse functional signals were observed in the NTEs, suggesting their biological implications. This study provides a comprehensive landscape of the shared and distinct characteristics of non-AUG translation initiation between monocot and dicot plants, and highlights the complexity of genetic decoding in shaping the dynamic composition of plant proteomes.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_20:43:53.981.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuqian Zhang
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: NEWT:4577; scientific name: Oryza sativa (Rice); NCBI TaxID: NEWT:4530; scientific name: Glycine max; NCBI TaxID: NEWT:3847;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-03 05:12:15	ID requested
⏵ 1	2026-04-26 20:43:55	announced

Zhang Y, Chang B, Wang S, Tian L, Zhao H, Luo W, Zhang H, Zhang S, Wu S, Wu L, Computational prediction-combined proteogenomics unveils widespread non-AUG translation initiation events in plants. Nucleic Acids Res, 54(7):(2026) [pubmed]

10.1093/nar/gkag303;

submitter keyword: non-AUG translation initiation

N-terminally extended protein isoforms

N-terminal extensions (NTEs)

upstream translation initiation sites (uTISs)

proteogenomics

monocots and dicots

Liuji Wu
contact affiliation	National Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, China
contact email	wlj200120@163.com
lab head
Yuqian Zhang
contact affiliation	Henan Agricultural university
contact email	yqzhang0825@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058528
     1. Label: PRIDE project
     2. Name: Computational prediction combined with proteogenomics unveils widespread non-AUG translation initiation events in plants