PXD058505
PXD058505 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Discovery of Calcineurin as an Essential Regulator of Sleep |
| Description | Research into mechanisms underlying sleep traditionally relies on electrophysiology and genetics. Because sleep can only be measured on whole animals by behavioral observations and physical means, no sleep research was initiated by biochemical and chemical biological approaches. We used phosphorylation sites of kinases important for sleep as targets for biochemical and chemical biological approaches. Sleep was increased in mice carrying a threonine-to-alanine substitution at residue T469 of salt inducible kinase 3 (SIK3). Our biochemical purification and photo-crosslinking revealed calcineurin dephosphorylation, both in vitro and in vivo, of SIK3 at T469 and S551, but not T221. Knocking down calcineurin regulatory subunit reduced daily sleep by more than 5 hours, exceeding all known mouse mutants. Our work uncovered a critical physiological role for calcineurin in sleep and pioneered biochemical purification and chemical biology as effective approaches to study sleep. |
| HostingRepository | iProX |
| AnnounceDate | 2024-12-02 |
| AnnouncementXML | Submission_2024-12-02_20:29:58.703.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chenxi Jia |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | phosphorylated residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-12-02 20:03:40 | ID requested | |
| ⏵ 1 | 2024-12-02 20:29:59 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: sleep regulation, calcineurin, SIK3, biochemical purification, photo-crosslinking |
Contact List
| Chenxi Jia | |
|---|---|
| contact affiliation | Beijing Proteome Research Center, National Center for Protein Sciences(Beijing) |
| contact email | cjia99@126.com |
| lab head | |
| Chenxi Jia | |
| contact affiliation | Beijing Proteome Research Center, National Center for Protein Sciences(Beijing) |
| contact email | cjia99@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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