PXD058487
PXD058487 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proximity labeling and orthogonal nanobody pulldown (ID-oPD) approaches to map the spinophilin interactome uncover a role for spinophilin in protein regulation. |
| Description | The major synaptic protein phosphatase 1 (PP1) targeting protein, spinophilin can inhibit or target PP1 activity towards diverse substrates. To detail the full panoply of substrates at which spinophilin modulates PP1 activity, we have validated a novel approach that permits proximity labeling and orthogonal nanobody pulldown (ID-oPD) of spinophilin protein complexes. We identified 614 specific and 217 selective spinophilin interacting proteins in a HEK293 overexpression system, many of which are involved in mRNA translation or metabolism. We validated a subset of these interactors using neutravidin and ALFA-tag nanobody pulldowns followed by immunoblotting. Additionally, using wildtype or an F451A mutant spinophilin that limits PP1 binding, we found that the PP1 binding-deficient spinophilin mutant enhanced its own expression, but limited the expression of proteins involved in mRNA translation and metabolism Moreover, in the striatum, spinophilin mRNA is highly neuropil localized and endogenous spinophilin limits its own expression. Overall, our studies demonstrate a novel role for spinophilin in mediating synaptic protein expression and that care must be taken to not over-control when using proximity labeling to detect PP1 targeting protein interactomes. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-07-18 |
| AnnouncementXML | Submission_2025-07-18_10:47:27.154.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Emma Doud |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Phospho |
| Instrument | Orbitrap Eclipse; instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-12-02 12:32:49 | ID requested | |
| ⏵ 1 | 2025-07-18 10:47:27 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: spinophilin, protein phosphatase 1, proximity labeling, orthogonal nanobody pulldown, proximity labeling and orthogonal nanobody pulldown (ID-oPD), ALFA-tag, TurboID, DatasetType:Proteomics |
Contact List
| Amber L. Mosley | |
|---|---|
| contact affiliation | Indiana University School of Medicine |
| contact email | almosley@iu.edu |
| lab head | |
| A.J. Baucum II | |
| contact affiliation | Indiana University School of Medicine |
| contact email | ajbaucum@iu.edu |
| lab head | |
| Emma Doud | |
| contact affiliation | IUSM |
| contact email | edoud@iu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v07/MSV000096575/ |
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