PXD058484 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mapping viral protease-mediated processing of astrovirus nonstructural polyprotein C4PR_LIV |
| Description | Many viruses with compact RNA genomes use a replication strategy involving the translation and proteolytic processing of a polyprotein. Astroviruses include a range of human and animal species and can infect cells of intestinal and neuronal origin. These viruses have positive-sense RNA genomes and translate their nonstructural polyprotein, which is subsequently processed by viral and cellular proteases. The polyprotein cleavage sites are predicted but not experimentally confirmed. Using N-terminomics and a complementary range of other experimental techniques, we mapped polyprotein cleavage sites in classical human astrovirus 1 (HAstV1) and neurotropic astrovirus strain MLB2, providing a map of individual proteins and their boundaries. Furthermore, we characterize processing precursors and replication properties of processing-defective mutant replicons, further elucidating the importance of polyprotein cleavage in astroviruses. Understanding polyprotein processing dynamics is essential for interpreting viral infection stages and identifying new drug targets and antiviral strategies. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-07 |
| AnnouncementXML | Submission_2026-06-07_15:36:35.270.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Leandro Xavier Neves |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Human astrovirus MLB2; NCBI TaxID: NEWT:1186059; scientific name: Human astrovirus 1; NCBI TaxID: NEWT:12456; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-12-02 10:45:25 | ID requested | |
| ⏵ 1 | 2026-06-07 15:36:35 | announced | |
Publication List
Keyword List
| submitter keyword: TMT-HUNTER, N-terminomics, astrovirus |
Contact List
| Ed Emmott |
|---|
| contact affiliation | Centre for Proteome Research, Dept. Biochemistry & Systems Biology, University of Liverpool, Liverpool, UK |
| contact email | e.emmott@liverpool.ac.uk |
| lab head | |
| Leandro Xavier Neves |
|---|
| contact affiliation | Centre for Proteome Research, Department of Biochemistry, Cell & Systems Biology, Institute of Systems, Molecular & Integrative Biology, University of Liverpool, Liverpool, UK |
| contact email | leandroxneves@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058484
- Label: PRIDE project
- Name: Mapping viral protease-mediated processing of astrovirus nonstructural polyprotein C4PR_LIV
