PXD058467

PXD058467 is an original dataset announced via ProteomeXchange.

Title	Isolation of lysosomal fractions from ES2 cancer cells
Description	Pro-metastatic proteins are notably stabilized in cancer cells to promote future development, and tumors frequently repeat programs to gain invasive and dispersion capabilities. It is uncertain if unsuccessful protein degradation has an impact on cancer's metastatic programs. It is still mostly unclear if CMA can exhibit degradative selectivity towards metastasis regulators, such as EMT-associated proteins, influencing the cancer cells' capacity to spread and develop further. We used ovarian carcinoma-derived ES2 cells, which exhibit strong genetic similarity to ovarian cancers with mesenchymal characteristics, in a multiplexed quantitative mass spectrometry (MS)-based proteomics technique to experimentally investigate and uncover CMA-targetable proteins causing EMT.
HostingRepository	PRIDE
AnnounceDate	2025-02-04
AnnouncementXML	Submission_2025-02-04_00:52:02.836.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Boxi Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-02 01:53:05	ID requested
⏵ 1	2025-02-04 00:52:03	announced

10.15252/EMMM.202419764;

submitter keyword: Lysosome LAMP2A

Helin Vakifahmetoglu
contact affiliation	Department of Physiology and Pharmacology, Karolinska Institutet
contact email	helin.norberg@ki.se
lab head
Boxi Zhang
contact affiliation	Karolinska Institutet
contact email	boxi.zhang@ki.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058467
     1. Label: PRIDE project
     2. Name: Isolation of lysosomal fractions from ES2 cancer cells