PXD058429

PXD058429 is an original dataset announced via ProteomeXchange.

Title	Unique Leishmania mexicana clones secrete populations of extracellular vesicles with unique protein profile and variable infectious capability
Description	The study of extracellular vesicles has become an incredibly important field of study, but the inherent heterogeneity of these vesicles continues to make their study challenging. The genetic variability and well-documented protocols for the growth and vesicle isolation from Leishmania parasites provide a unique opportunity to compare the heterogeneity of different populations secreted by Leishmania clones. Leishmania mexicana was cultured on solid SDM agar plates and 8 clonal colonies were selected. The EVs collected from the liquid cultures of these 8 clones were assessed by NTA, TEM, and proteomic analysis. We found that all 8 clonal L. mexicana cultures were visually indistinguishable from each other and had similar growth rate, and these physical similarities extended to their EVs. However, proteomic analysis reveals that the EVs collected have unique protein profiles compared to each other and EVs isolated from a heterogeneous liquid culture of L. mexicana. We selected 3 clonal EVs for further mouse infection experiments and found that EVs from CL7 L. mexicana consistently caused reduced footpad swelling in C57BL6 mice footpads compared to EVs from CL1, CL8, and heterogenous L. mexicana. This trend was not observed when infecting Balb/C mice with the parasites alone, with only CL1 L. mexicana causing significantly increased infection, nor in C57BL6 mice with all clonal L. mexicana parasites causing a similar infection. Our results together show that EVs isolated from different clonal colonies of L. mexicana have distinct differences in protein cargo which can lead to varying outcomes on Leishmania infection. Further evaluation will be needed to determine the underlying mechanisms behind this and verify that differences observed in infectivity are directly caused by variations between our L. mexicana clones, especially genetic sequencing and immunoblotting to validate our results.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_05:28:02.073.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058429
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	George Dong
SpeciesList	scientific name: Leishmania mexicana; NCBI TaxID: 5665;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-29 15:47:14	ID requested
⏵ 1	2025-05-07 05:28:02	announced

10.3389/fcimb.2024.1443262;

10.6019/PXD058429;

Dong G, Douanne N, Fernandez-Prada C, Olivier M, clones secrete populations of extracellular vesicles with unique protein profile and variable infectious capability. Front Cell Infect Microbiol, 14():1443262(2024) [pubmed]

submitter keyword: exosomes, proteomics, extracellular vesicles, infectious disease,Leismania mexicana, clonal cultures

Martin Olivier
contact affiliation	Research Institute of McGill University Health Centre
contact email	martin.olivier@mcgill.ca
lab head
George Dong
contact affiliation	Research Institute of McGill University Health Centre
contact email	george.dong@mail.mcgill.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058429

PRIDE project URI

• PRIDE
  1. PXD058429
     1. Label: PRIDE project
     2. Name: Unique Leishmania mexicana clones secrete populations of extracellular vesicles with unique protein profile and variable infectious capability