⮝ Full datasets listing

PXD058429

PXD058429 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUnique Leishmania mexicana clones secrete populations of extracellular vesicles with unique protein profile and variable infectious capability
DescriptionThe study of extracellular vesicles has become an incredibly important field of study, but the inherent heterogeneity of these vesicles continues to make their study challenging. The genetic variability and well-documented protocols for the growth and vesicle isolation from Leishmania parasites provide a unique opportunity to compare the heterogeneity of different populations secreted by Leishmania clones. Leishmania mexicana was cultured on solid SDM agar plates and 8 clonal colonies were selected. The EVs collected from the liquid cultures of these 8 clones were assessed by NTA, TEM, and proteomic analysis. We found that all 8 clonal L. mexicana cultures were visually indistinguishable from each other and had similar growth rate, and these physical similarities extended to their EVs. However, proteomic analysis reveals that the EVs collected have unique protein profiles compared to each other and EVs isolated from a heterogeneous liquid culture of L. mexicana. We selected 3 clonal EVs for further mouse infection experiments and found that EVs from CL7 L. mexicana consistently caused reduced footpad swelling in C57BL6 mice footpads compared to EVs from CL1, CL8, and heterogenous L. mexicana. This trend was not observed when infecting Balb/C mice with the parasites alone, with only CL1 L. mexicana causing significantly increased infection, nor in C57BL6 mice with all clonal L. mexicana parasites causing a similar infection. Our results together show that EVs isolated from different clonal colonies of L. mexicana have distinct differences in protein cargo which can lead to varying outcomes on Leishmania infection. Further evaluation will be needed to determine the underlying mechanisms behind this and verify that differences observed in infectivity are directly caused by variations between our L. mexicana clones, especially genetic sequencing and immunoblotting to validate our results.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_05:28:02.073.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD058429
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterGeorge Dong
SpeciesList scientific name: Leishmania mexicana; NCBI TaxID: 5665;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-11-29 15:47:14ID requested
12025-05-07 05:28:02announced
Publication List
10.3389/fcimb.2024.1443262;
10.6019/PXD058429;
Dong G, Douanne N, Fernandez-Prada C, Olivier M, clones secrete populations of extracellular vesicles with unique protein profile and variable infectious capability. Front Cell Infect Microbiol, 14():1443262(2024) [pubmed]
Keyword List
submitter keyword: exosomes, proteomics, extracellular vesicles, infectious disease,Leismania mexicana, clonal cultures
Contact List
Martin Olivier
contact affiliationResearch Institute of McGill University Health Centre
contact emailmartin.olivier@mcgill.ca
lab head
George Dong
contact affiliationResearch Institute of McGill University Health Centre
contact emailgeorge.dong@mail.mcgill.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058429
PRIDE project URI
Repository Record List
[ + ]