PXD058427

PXD058427 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of infected macrophages reveals novel Leishmania virulence factors
Description	Leishmaniasis is a major public health problem, causing diseases ranging from self-healing skin lesions to life-threatening chronic infections. Understanding how Leishmania parasites evade the host defense system is crucial for understanding the different manifestations of the disease and for improving diagnostic tools and drug development. We performed high-resolution proteome profiling of Leishmania spp. across three species during macrophage infection and identified distinct temporal expression patterns. Clustering analysis revealed unique protein expression profiles for each Leishmania species, whereas pairwise enrichment analysis revealed specific up- and downregulation patterns at different infection stages. Our results confirmed known virulence factors and highlighted new ones, demonstrating how our dataset could be used. We validated the dataset by showing that deletion of putative L. mexicana virulence factors resulted in reduced stage differentiation capacity and infectivity. This novel Leishmania infectome database (LInfDB) is available online for further exploration (https://butterlab.imb-mainz.de/LInfDB), providing a valuable resource for future research.
HostingRepository	PRIDE
AnnounceDate	2026-01-28
AnnouncementXML	Submission_2026-01-28_12:57:49.978.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Falk Butter
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: Leishmania infantum; NCBI TaxID: NEWT:5671; scientific name: Leishmania major; NCBI TaxID: NEWT:5664; scientific name: Leishmania mexicana mexicana; NCBI TaxID: NEWT:44270;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-29 14:45:48	ID requested
⏵ 1	2026-01-28 12:57:50	announced

Dataset with its publication pending

submitter keyword: None

Falk Butter
contact affiliation	IMVZ, Friedrich-Loeffler-Institut, Greifswald
contact email	falk.butter@fli.de
lab head
Falk Butter
contact affiliation	IMVZ, Friedrich-Loeffler-Institute
contact email	falk.butter@fli.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD058427

PRIDE project URI

• PRIDE
  1. PXD058427
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of infected macrophages reveals novel Leishmania virulence factors