PXD058403

PXD058403 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic identification of MAVS carboxylation site–associated proteins
Description	To identify proteins that differentially interact with carboxylated and non-carboxylated MAVS, we overexpressed Flag-tagged WT or 4A mutant (D53A, E70A, E80A, and D83A) forms of hMAVS in 293T cells and analyzed the interactors using immunoprecipitation followed by mass spectrometry (MS). Cell lysates were subjected to immunoprecipitation with anti-FLAG antibodies, and the immunoprecipitated proteins were subsequently analyzed by LC-MS/MS.
HostingRepository	jPOST
AnnounceDate	2025-07-23
AnnouncementXML	Submission_2025-07-22_17:34:18.824.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tomohiko Okazaki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	unknown modification; L-methionine sulfoxide
Instrument	QSTAR

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-28 23:11:09	ID requested
⏵ 1	2025-07-22 17:34:20	announced

Publication List

Okazaki T, Nozaki K, Morimoto N, Otobe Y, Saito R, Abe S, Okajima M, Yoshitane H, Hatta T, Iemura SI, Natsume T, Kosako H, Yamasaki M, Inoue S, Kondo T, Koseki H, Gotoh Y, Membrane topology inversion of GGCX mediates cytoplasmic carboxylation for antiviral defense. Science, 389(6755):84-91(2025) [pubmed]

Okazaki T, Nozaki K, Morimoto N, Otobe Y, Saito R, Abe S, Okajima M, Yoshitane H, Hatta T, Iemura SI, Natsume T, Kosako H, Yamasaki M, Inoue S, Kondo T, Koseki H, Gotoh Y, Membrane topology inversion of GGCX mediates cytoplasmic carboxylation for antiviral defense. Science, 389(6755):84-91(2025) [pubmed]

Keyword List

submitter keyword: mitochondria, innate immunity

submitter keyword: mitochondria, innate immunity

Contact List

Tomohiko Okazaki
lab head
Tomohiko Okazaki
contact affiliation	Hokkaido University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003494/