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PXD058393

PXD058393 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProvirus deletion from Haloferax volcanii affects motility, stress resistance and CRISPR RNA expression
DescriptionHaloferax volcanii harbours four putative proviruses: Halfvol1, Halfvol2, Halfvol3 and Halfvol4. In this study we successfully deleted all four provirus genomes, demonstrating, that they are not essential. Transcriptome comparison between this strain (∆Halfvol1-4) and a wild type strain reveals an increase in archaella and chemotaxis gene expression, resulting in higher swarming motility in ∆Halfvol1-4. Furthermore, ∆Halfvol1-4 cells show an elongated cell shape and a higher resistance to H2O2 stress compared to the wild type. RNA-seq also revealed down-regulation of CRISPR arrays in the provirus-free strain. Circularised genomes of Halfvol1, Halfvol2 and Halfvol3 were found in the culture supernatant. This confirms excision of the proviruses from the chromosome, which seems to happen more efficiently at low temperature (30°C). Electron microscopy revealed potential viral particles in the supernatant, and mass spectrometry analysis confirmed the presence of structural viral proteins of Halfvol1 and Halfvol3 in the isolated virus sample. These observations suggest that these proviruses are active and cause a chronic infection in Hfx. volcanii.
HostingRepositoryPRIDE
AnnounceDate2025-05-26
AnnouncementXMLSubmission_2025-05-25_16:29:04.107.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOlexandr Dybkov
SpeciesList scientific name: Haloferax volcanii (Halobacterium volcanii); NCBI TaxID: 2246;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-11-28 15:34:26ID requested
12025-05-25 16:29:05announced
Publication List
10.1093/femsml/uqaf008;
Di Cianni N, Bolsinger S, Brendel J, Raabe M, K, ö, nig S, Mitchell L, Bischler T, Gr, ä, fenhan T, Read C, Erdmann S, Allers T, Walther P, Urlaub H, Dyall-Smith M, Pfeiffer F, Marchfelder A, affects motility, stress resistance, and CRISPR RNA expression. Microlife, 6():uqaf008(2025) [pubmed]
Keyword List
submitter keyword: provirus,Haloferax volcanii, virus, motility, archaea, CRISPR RNA
Contact List
Henning Urlaub
contact affiliationBioanalytical Mass Spectrometry Group, Max Planck Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077 Goettingen, Germany
contact emailhenning.urlaub@mpinat.mpg.de
lab head
Olexandr Dybkov
contact affiliationMax Planck Institute for Multidisciplinary Sciences
contact emailodybkov@gwdg.de
dataset submitter
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Dataset FTP location
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