PXD058393

PXD058393 is an original dataset announced via ProteomeXchange.

Title	Provirus deletion from Haloferax volcanii affects motility, stress resistance and CRISPR RNA expression
Description	Haloferax volcanii harbours four putative proviruses: Halfvol1, Halfvol2, Halfvol3 and Halfvol4. In this study we successfully deleted all four provirus genomes, demonstrating, that they are not essential. Transcriptome comparison between this strain (∆Halfvol1-4) and a wild type strain reveals an increase in archaella and chemotaxis gene expression, resulting in higher swarming motility in ∆Halfvol1-4. Furthermore, ∆Halfvol1-4 cells show an elongated cell shape and a higher resistance to H2O2 stress compared to the wild type. RNA-seq also revealed down-regulation of CRISPR arrays in the provirus-free strain. Circularised genomes of Halfvol1, Halfvol2 and Halfvol3 were found in the culture supernatant. This confirms excision of the proviruses from the chromosome, which seems to happen more efficiently at low temperature (30°C). Electron microscopy revealed potential viral particles in the supernatant, and mass spectrometry analysis confirmed the presence of structural viral proteins of Halfvol1 and Halfvol3 in the isolated virus sample. These observations suggest that these proviruses are active and cause a chronic infection in Hfx. volcanii.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_16:29:04.107.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olexandr Dybkov
SpeciesList	scientific name: Haloferax volcanii (Halobacterium volcanii); NCBI TaxID: 2246;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-28 15:34:26	ID requested
⏵ 1	2025-05-25 16:29:05	announced

10.1093/femsml/uqaf008;

Di Cianni N, Bolsinger S, Brendel J, Raabe M, K, ö, nig S, Mitchell L, Bischler T, Gr, ä, fenhan T, Read C, Erdmann S, Allers T, Walther P, Urlaub H, Dyall-Smith M, Pfeiffer F, Marchfelder A, affects motility, stress resistance, and CRISPR RNA expression. Microlife, 6():uqaf008(2025) [pubmed]

submitter keyword: provirus,Haloferax volcanii, virus, motility, archaea, CRISPR RNA

Henning Urlaub
contact affiliation	Bioanalytical Mass Spectrometry Group, Max Planck Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077 Goettingen, Germany
contact email	henning.urlaub@mpinat.mpg.de
lab head
Olexandr Dybkov
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	odybkov@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058393
     1. Label: PRIDE project
     2. Name: Provirus deletion from Haloferax volcanii affects motility, stress resistance and CRISPR RNA expression