PXD058393 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Provirus deletion from Haloferax volcanii affects motility, stress resistance and CRISPR RNA expression |
Description | Haloferax volcanii harbours four putative proviruses: Halfvol1, Halfvol2, Halfvol3 and Halfvol4. In this study we successfully deleted all four provirus genomes, demonstrating, that they are not essential. Transcriptome comparison between this strain (∆Halfvol1-4) and a wild type strain reveals an increase in archaella and chemotaxis gene expression, resulting in higher swarming motility in ∆Halfvol1-4. Furthermore, ∆Halfvol1-4 cells show an elongated cell shape and a higher resistance to H2O2 stress compared to the wild type. RNA-seq also revealed down-regulation of CRISPR arrays in the provirus-free strain. Circularised genomes of Halfvol1, Halfvol2 and Halfvol3 were found in the culture supernatant. This confirms excision of the proviruses from the chromosome, which seems to happen more efficiently at low temperature (30°C). Electron microscopy revealed potential viral particles in the supernatant, and mass spectrometry analysis confirmed the presence of structural viral proteins of Halfvol1 and Halfvol3 in the isolated virus sample. These observations suggest that these proviruses are active and cause a chronic infection in Hfx. volcanii. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-26 |
AnnouncementXML | Submission_2025-05-25_16:29:04.107.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Olexandr Dybkov |
SpeciesList | scientific name: Haloferax volcanii (Halobacterium volcanii); NCBI TaxID: 2246; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-11-28 15:34:26 | ID requested | |
⏵ 1 | 2025-05-25 16:29:05 | announced | |
Publication List
10.1093/femsml/uqaf008; |
Di Cianni N, Bolsinger S, Brendel J, Raabe M, K, ö, nig S, Mitchell L, Bischler T, Gr, ä, fenhan T, Read C, Erdmann S, Allers T, Walther P, Urlaub H, Dyall-Smith M, Pfeiffer F, Marchfelder A, affects motility, stress resistance, and CRISPR RNA expression. Microlife, 6():uqaf008(2025) [pubmed] |
Keyword List
submitter keyword: provirus,Haloferax volcanii, virus, motility, archaea, CRISPR RNA |
Contact List
Henning Urlaub |
contact affiliation | Bioanalytical Mass Spectrometry Group, Max Planck Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077 Goettingen, Germany |
contact email | henning.urlaub@mpinat.mpg.de |
lab head | |
Olexandr Dybkov |
contact affiliation | Max Planck Institute for Multidisciplinary Sciences |
contact email | odybkov@gwdg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058393 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058393
- Label: PRIDE project
- Name: Provirus deletion from Haloferax volcanii affects motility, stress resistance and CRISPR RNA expression