PXD058373

PXD058373 is an original dataset announced via ProteomeXchange.

Title	A translational protocol for the analysis of plasma-derived extracellular vesicle proteomics
Description	We establish here an EV purification and proteomic analysis workflow in a real world clinical setting in which we evaluate different variables, from blood collection through protein preparation for mass spectrometry (MS). We compare blood collection tubes, sample transportation and storage conditions, EV enrichment methods, size exclusion chromatography columns, as well as lysis and protein preparation conditions. For each parameter tested, we assess hemolysis, particle concentration and size, protein quantity, protein markers (EV-related, cell-specific, co-isolates) and, for some, comprehensive proteomic analysis using mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:20:20.134.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katharina Clemm von Hohenberg
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-28 02:59:38	ID requested
⏵ 1	2025-07-13 16:20:20	announced

10.1038/s41598-025-08366-8;

R, í, os de Los R, í, os Res, é, ndiz J, Herrmann-Sim F, Wilkesmann L, Helm D, Schneider M, Campione G, Pl, ü, gge K, Greiner G, Lazaro Garc, í, a L, Berker J, Richter K, Zielske L, Hofmann WK, Clemm von Hohenberg K, A translational protocol optimizes the isolation of plasma-derived extracellular vesicle proteomics. Sci Rep, 15(1):24292(2025) [pubmed]

submitter keyword: protocol, proteomics, preanalytics, liquid biopsy, plasma,Extracellular vesicles, translation

Dr. med. Katharina Clemm von Hohenberg
contact affiliation	German Cancer Research Center (DKFZ) Heidelberg
contact email	k.clemm@dkfz-heidelberg.de
lab head
Katharina Clemm von Hohenberg
contact affiliation	Junior Clinical Cooperation Unit Translational Lymphoma Research German Cancer Research Center Heidelberg & Medical Faculty Mannheim, Heidelberg University
contact email	k.clemm@dkfz-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD058373

PRIDE project URI

• PRIDE
  1. PXD058373
     1. Label: PRIDE project
     2. Name: A translational protocol for the analysis of plasma-derived extracellular vesicle proteomics