PXD058370

PXD058370 is an original dataset announced via ProteomeXchange.

Title	GALNT11 Controlled O-glycans Modulate Ligand Interactions of the Endocytic Receptor LRP1
Description	Low-density lipoprotein receptor (LDLR) and LDLR-related proteins (LRPs) are essential endocytic receptors and regulators of multiple physiological processes including protein reabsorption, cholesterol clearance, and neuronal protein trafficking. Previously, we identified O-GalNAc glycans within ligand-binding regions of LRP receptors, with functional implications for the uptake of LDL (via LDLR) and albumin (via LRP2). Notably, these O-glycans are specifically introduced by GALNT11, one of 20 GALNT isoenzymes. Here, we used cell models to investigate the role of GALNT11-mediated glycosylation in modulating ligand interactions of the widely expressed receptor LRP1. We find that while GALNT11-specific glycosylation does not alter LRP1-mediated uptake of ligands such as ApoE and RAP, it significantly affects the uptake of tau, amyloid beta and Hemopexin. We further show that GALNT11 O-glycans on LRP1 are selectively hyposialylated, and molecular dynamics simulations reveal that their sialylation can prevent glycan-ligand interactions.
HostingRepository	PRIDE
AnnounceDate	2026-03-02
AnnouncementXML	Submission_2026-03-02_01:47:00.968.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergey Vakhrushev
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-28 02:03:42	ID requested
⏵ 1	2026-03-02 01:47:01	announced

Dataset with its publication pending

submitter keyword: Glycosylation,LRP1, TMT, mass spectrometry, GALNT11

Sergey Vakhrushev
contact affiliation	Department of Cellular and Molecular Medicine, University of Copenhagen
contact email	seva@sund.ku.dk
lab head
Sergey Vakhrushev
contact affiliation	Department of Cellular and Molecular Medicine
contact email	seva@sund.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058370
     1. Label: PRIDE project
     2. Name: GALNT11 Controlled O-glycans Modulate Ligand Interactions of the Endocytic Receptor LRP1