PXD058368

PXD058368 is an original dataset announced via ProteomeXchange.

Title	Bacterial effectors mediate kinase reprogramming through a series of evolutionary conserved amino acids that mimic eukaryotic motifs
Description	Bacteria have evolved numerous biochemical processes that underpin their biology and pathogenesis. The small, non-enzymatic bacterial (Salmonella) effector SteE mediates kinase reprogramming, whereby the canonical serine/threonine host kinase GSK3 gains tyrosine-directed activity towards neosubstrates, promoting Salmonella virulence. Yet, both the mechanism behind the switch in GSK3’s activity and the diversity of this phenomenon remain to be determined. Here we show that kinase reprogramming of GSK3 is mediated by putative homologues from diverse Gram-negative pathogens. Next, we identify both the molecular basis of how SteE targets GSK3 and then uncover that the SteE-induced tyrosine activity conferred on GSK3 occurs by mimicry of an L/xGxP motif, previously shown to mediate GSK3 autophosphorylation on a tyrosine. Together, we demonstrate how a family of intrinsically disordered proteins mediate kinase reprogramming through the molecular mimicry of eukaryotic short linear motifs. With these advances comes the potential for the rationale design of synthetic reprogramming proteins
HostingRepository	PRIDE
AnnounceDate	2025-04-25
AnnouncementXML	Submission_2025-04-25_05:56:49.091.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Maslen
SpeciesList	scientific name: Salmonella sp. WT; NCBI TaxID: 1077200;
ModificationList	No PTMs are included in the dataset
Instrument	SELECT SERIES Cyclic IMS

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-28 01:07:14	ID requested
⏵ 1	2025-04-25 05:56:49	announced

Dataset with its publication pending

submitter keyword: Mass Spectrometry, HDX,Kinase

Dr. Katrin Rittinger
contact affiliation	Molecular Structure of Cell Signalling Laboratory, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK
contact email	katrin.rittinger@crick.ac.uk
lab head
Sarah Maslen
contact affiliation	The Francis Crick Institute
contact email	sarah.maslen@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058368
     1. Label: PRIDE project
     2. Name: Bacterial effectors mediate kinase reprogramming through a series of evolutionary conserved amino acids that mimic eukaryotic motifs