PXD058345

PXD058345 is an original dataset announced via ProteomeXchange.

Title	Blobby is a synaptic active zone assembly protein required for memory in Drosophila
Description	At presynaptic active zones (AZs), scaffold proteins are critical for coordinating synaptic vesicle (SV) release and forming essential nanoarchitectures. However, regulatory principles steering AZ scaffold assembly, function, and plasticity remain insufficiently understood. We here identify a novel Drosophila AZ protein, "Blobby", essential for proper AZ nano-organization. Blobby biochemically associates with the ELKS family AZ scaffold protein Bruchpilot (BRP) and integrates into newly forming AZs. Loss of Blobby results in fewer AZs forming, ectopic AZ scaffold protein accumulations ("blobs") and disrupts nanoscale architecture of the BRP-AZ scaffold. Functionally, blobby mutants show diminished evoked synaptic currents due to reduced SV release probability and fewer functional SV release sites. Blobby is also present in adult brain synapses, and post-developmental knockdown of Blobby in the mushroom body impairs olfactory aversive memory consolidation. Thus, our analysis identifies a new layer of AZ regulation critical for developmental AZ assembly but also for AZ-mediated plasticity controlling behavior.
HostingRepository	PRIDE
AnnounceDate	2025-03-17
AnnouncementXML	Submission_2025-03-17_07:57:10.291.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Max Ruwolt
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-27 11:03:57	ID requested
⏵ 1	2025-03-17 07:57:10	announced

10.1038/S41467-024-55382-9;

submitter keyword: synapse,drosophila, bruchpilot

Stephan Sigrist
contact affiliation	Freie Universität Berlin
contact email	stephan.sigrist@fu-berlin.de
lab head
Max Ruwolt
contact affiliation	Leibniz-Forschungsinstitut for Molecular Pharmacology
contact email	ruwolt@fmp-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058345
     1. Label: PRIDE project
     2. Name: Blobby is a synaptic active zone assembly protein required for memory in Drosophila