PXD058335 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | CEPD/ROXY6-9 proteins activate N starvation response genes by interfering with the repressive activity of transcription factors TGA1 and TGA4 |
Description | Fluctuations in the supply of soil nitrogen (N) cause profound alterations in the transcriptome of plants, allowing improved N acquisition, allocation and remobilization. In Arabidopsis thaliana, N starvation-induced CEPD (C-TERMINALLY ENCODED PEPTIDE DOWNSTREAM) proteins are required for transcriptional activation of genes involved in efficient nitrate uptake by roots. CEPDs belong to a plant-specific class of glutaredoxin-like proteins (called ROXYs in A. thaliana) that interact with TGACG-binding transcription factors (TGAs). Here we show that TGA1 and TGA4 act as molecular links between CEPDs/ROXY6-9 and target promoters in roots. In the absence of CEPDs/ROXYs 6-9, TGA1/4 recruit a strong repressive complex. In wild-type plants, this repressive complex is dysfunctional and transcriptional activation upon N starvation is most likely regulated by the increased ratio of CEPDs/ROXYs 6-9 over antagonistically acting ROXYs encoding the TOPLESS-interacting ALWL motif. TGA1/4 are required for transcriptional repression on full N supply and/or for transcriptional activation under N starvation, depending on the promoter and on the exact composition of the medium. Although CEPDs/ROXY6-9 resemble glutaredoxins with glutathione-dependent oxidoreductase activity, complementation analysis with ROXY9 variants encoding mutations in the active site excludes that ROXY9 functions by altering the redox state of target proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_05:29:12.748.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Corinna Thurow |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-11-27 08:05:00 | ID requested | |
⏵ 1 | 2025-05-07 05:29:13 | announced | |
Publication List
10.1093/plcell/koaf038; |
Thurow C, Pelizaeus AM, Mrozek P, Ho, ß, bach BM, Budimir J, Schmitt K, Valerius O, Braus G, Gatz C, Redox-inactive CC-type glutaredoxins interfere with TGA transcription factor-dependent repression of target promoters in roots. Plant Cell, 37(3):(2025) [pubmed] |
Keyword List
submitter keyword: TGA transcription factors, TurboID-mediated proximity labeling,Arabidopsis, N starvation, CC-type glutaredoxins |
Contact List
Christiane Gatz |
contact affiliation | Georg-August-University Goettingen, Albrecht-von-Haller-Institute of Plant Sciences, Department of Plant Molecular Biology and Physiology |
contact email | cgatz@gwdg.de |
lab head | |
Corinna Thurow |
contact affiliation | Georg-August-University Goettingen, Albrecht-von-Haller-Institute of Plant Sciences, Department of Plant Molecular Biology and Physiology |
contact email | cthurow@uni-goettingen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058335
- Label: PRIDE project
- Name: CEPD/ROXY6-9 proteins activate N starvation response genes by interfering with the repressive activity of transcription factors TGA1 and TGA4