PXD058335

PXD058335 is an original dataset announced via ProteomeXchange.

Title	CEPD/ROXY6-9 proteins activate N starvation response genes by interfering with the repressive activity of transcription factors TGA1 and TGA4
Description	Fluctuations in the supply of soil nitrogen (N) cause profound alterations in the transcriptome of plants, allowing improved N acquisition, allocation and remobilization. In Arabidopsis thaliana, N starvation-induced CEPD (C-TERMINALLY ENCODED PEPTIDE DOWNSTREAM) proteins are required for transcriptional activation of genes involved in efficient nitrate uptake by roots. CEPDs belong to a plant-specific class of glutaredoxin-like proteins (called ROXYs in A. thaliana) that interact with TGACG-binding transcription factors (TGAs). Here we show that TGA1 and TGA4 act as molecular links between CEPDs/ROXY6-9 and target promoters in roots. In the absence of CEPDs/ROXYs 6-9, TGA1/4 recruit a strong repressive complex. In wild-type plants, this repressive complex is dysfunctional and transcriptional activation upon N starvation is most likely regulated by the increased ratio of CEPDs/ROXYs 6-9 over antagonistically acting ROXYs encoding the TOPLESS-interacting ALWL motif. TGA1/4 are required for transcriptional repression on full N supply and/or for transcriptional activation under N starvation, depending on the promoter and on the exact composition of the medium. Although CEPDs/ROXY6-9 resemble glutaredoxins with glutathione-dependent oxidoreductase activity, complementation analysis with ROXY9 variants encoding mutations in the active site excludes that ROXY9 functions by altering the redox state of target proteins.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_05:29:12.748.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Corinna Thurow
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-27 08:05:00	ID requested
⏵ 1	2025-05-07 05:29:13	announced

10.1093/plcell/koaf038;

Thurow C, Pelizaeus AM, Mrozek P, Ho, ß, bach BM, Budimir J, Schmitt K, Valerius O, Braus G, Gatz C, Redox-inactive CC-type glutaredoxins interfere with TGA transcription factor-dependent repression of target promoters in roots. Plant Cell, 37(3):(2025) [pubmed]

submitter keyword: TGA transcription factors, TurboID-mediated proximity labeling,Arabidopsis, N starvation, CC-type glutaredoxins

Christiane Gatz
contact affiliation	Georg-August-University Goettingen, Albrecht-von-Haller-Institute of Plant Sciences, Department of Plant Molecular Biology and Physiology
contact email	cgatz@gwdg.de
lab head
Corinna Thurow
contact affiliation	Georg-August-University Goettingen, Albrecht-von-Haller-Institute of Plant Sciences, Department of Plant Molecular Biology and Physiology
contact email	cthurow@uni-goettingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058335
     1. Label: PRIDE project
     2. Name: CEPD/ROXY6-9 proteins activate N starvation response genes by interfering with the repressive activity of transcription factors TGA1 and TGA4