PXD058329

PXD058329 is an original dataset announced via ProteomeXchange.

Title	Awakening of the RuMP cycle for partial methylotrophy in the thermophile Parageobacillus thermoglucosidasius
Description	Given sustainability and scalability concerns of using sugar feedstocks for microbial bioproduction of bulk chemicals, widening the feedstock range for microbial cell factories is of high interest. Methanol is a one-carbon alcohol that stands out as an alternative feedstock for the bioproduction of chemicals, as it is electron-rich, water-miscible and can be produced from several renewable resources. Bioconversion of methanol into products under thermophilic conditions (>50 °C) could be highly advantageous for industrial biotechnology. Although progress is being made with natural, thermophilic methylotrophic microorganisms, they are not yet optimal for bioproduction and establishing alternative thermophilic methylotrophic bioproduction platforms can widen possibilities. Hence, we set out to implement methanol assimilation in the emerging thermophilic model organism Parageobacillus thermoglucosidasius. We engineered P. thermoglucosidasius to be strictly dependent for its growth on methanol assimilation via the core of the highly efficient ribulose monophosphate (RuMP) cycle, while co-assimilating ribose. Surprisingly, this did not require heterologous expression of RuMP enzymes. Instead, by laboratory evolution we awakened latent, native enzyme activities to form the core of the RuMP cycle. We obtained fast methylotrophic growth in which ∼17 % of biomass was strictly obtained from methanol. This work lays the foundation for developing a versatile thermophilic bioproduction platform based on renewable methanol.
HostingRepository	PRIDE
AnnounceDate	2025-06-16
AnnouncementXML	Submission_2025-06-16_00:59:26.660.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Parageobacillus thermantarcticus; NCBI TaxID: 186116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-27 06:43:06	ID requested
⏵ 1	2025-06-16 00:59:27	announced

Paredes-Barrada M, Mathissen A, van der Molen RA, Jim, é, nez-Huesa PJ, Polano ME, Donati S, Abele M, Ludwig C, van Kranenburg R, Claassens NJ, Awakening of the RuMP cycle for partial methylotrophy in the thermophile Parageobacillus thermoglucosidasius. Metab Eng, 91():145-157(2025) [pubmed]

10.1016/j.ymben.2025.04.002;

submitter keyword: synthetic methylotrophy, thermophile, RuMP cycle,P. thermoglucosidasius, methanol dehydrogenase

Christina Ludwig
contact affiliation	Technical University of Munich (TUM); TUM School of Life Sciences, Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS); Gregor-Mendel-Strasse 4 85354 Freising GERMANY
contact email	tina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058329
     1. Label: PRIDE project
     2. Name: Awakening of the RuMP cycle for partial methylotrophy in the thermophile Parageobacillus thermoglucosidasius