PXD058328

PXD058328 is an original dataset announced via ProteomeXchange.

Title	Ocular Inoculation of Toad Venom: Toxic Cataract and Proteomic Profiling
Description	Purpose: To report a singular case of cataract caused by toad venom inoculation and to scrutinize the pathological mechanisms through proteomic sequencing of the lens specimen. Methods: A young Chinese male presented with progressively deteriorating vision in his right eye subsequent to a history of toad venom inoculation. He was diagnosed with a toxic cataract, and underwent phacoemulsification cataract surgery. Anterior capsule, nucleus, and cortex specimens from the patient (designated as PT_CAP, PT_PHACO, and PT_CTX, respectively) and age-related cataract controls (C_CAP, C_PHACO, and C_CTX, respectively) were collected and subjected to 4D label-free quantitative proteomics. Results: A multitude of differentially expressed proteins (DEPs) were identified in the patient's lens compared to those in the controls. Specifically, a total of 204 DEPs were identified in PT_CAP compared to C_CAP, with MYH6, MYL2, MYL3, STAT1, and ANK1 among the foremost regulated DEPs. The DEPs of PT_CAP were principally affiliated with functions including "transportation of small molecules", "regulation of metal ion transport", and "import into cell". A sum of 109 DEPs were delineated in PT_CTX compared to C_CTX, with TPM1 among the top-10 downregulated DEPs. Ninety-five DEPs were pinpointed in PT_PHACO compared to C_PHACO, with hexokinase among the top 10 downregulated DEPs. These proteins were ascertained to be linked with Na+/K+-ATPase activity. Conclusion: This study introduced the first documented case of toxic cataract caused by toad venom inoculation. Proteomic sequencing indicated a correlation between cataract and alterations in Na+/K+-ATPase activity, providing insights for the clinical management of ocular toad venom inoculation in subsequent cases.
HostingRepository	PRIDE
AnnounceDate	2025-05-13
AnnouncementXML	Submission_2025-05-13_06:10:00.464.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Danni Lyu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-27 06:22:35	ID requested
⏵ 1	2025-05-13 06:10:01	announced

10.3389/fmed.2024.1537770;

Lyu D, Ni S, Xu J, Zhu S, Xu JW, Feng Y, Shi C, Xu W, Ocular inoculation of toad venom: toxic cataract and proteomic profiling. Front Med (Lausanne), 11():1537770(2024) [pubmed]

submitter keyword: Toad venom

venom inoculation

toxic cataract

4D label-free quantitative proteomics

Na+/K+-ATPase

Wen Xu
contact affiliation	Eye Center of the 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China
contact email	xuwen2003@zju.edu.cn
lab head
Danni Lyu
contact affiliation	Eye Center of the 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China
contact email	elyon9106@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058328
     1. Label: PRIDE project
     2. Name: Ocular Inoculation of Toad Venom: Toxic Cataract and Proteomic Profiling