PXD058323

PXD058323 is an original dataset announced via ProteomeXchange.

Title	HepG2-derived Cell Membrane Vesicle Camouflaged Artificial Cells
Description	The integration of artificial and mammalian cells into semi-synthetic aggregates remains a challenge in bottom-up synthetic biology. Here, we demonstrate the fabrication of cell membrane vesicles (CMV) from HepG2 cells and their use as coating for alginate microgels to produce camouflaged artificial cells (ACs). These ACs are used for the assembly of either synthetic aggregates or semi-synthetic aggregates. On the one hand, a predator-defendant and a liver-like synthetic aggregates are investigated, showing promising initial steps towards complex synthetic aggregates. On the other hand, the camouflaged ACs show enhanced integration with HepG2 cells. The encapsulation of a reactive oxygen species (ROS) scavenger artificial enzyme in the ACs shows protection against tert-butyl hydroperoxide in terms of HepG2 cell viability, proliferation, and mitochondrial health in semisynthetic aggregates. Taken together, this effort is a step forward in combining both mammalian cells and ACs in the same aggregate where the latter act as support unit.
HostingRepository	PRIDE
AnnounceDate	2025-02-19
AnnouncementXML	Submission_2025-02-18_23:23:41.612.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fatih Demir
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-27 05:09:06	ID requested
⏵ 1	2025-02-18 23:23:41	announced

Dataset with its publication pending

submitter keyword: Human, CMVs, Liver, ACs, HepG2

Markus M. Rinschen
contact affiliation	Aarhus University Department of Biomedicine DK-8000C Aarhus Denmark
contact email	rinschen@biomed.au.dk
lab head
Fatih Demir
contact affiliation	Aarhus University Department of Biomedicine Markus Rinschen Lab
contact email	fatih.demir@biomed.au.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD058323

PRIDE project URI

• PRIDE
  1. PXD058323
     1. Label: PRIDE project
     2. Name: HepG2-derived Cell Membrane Vesicle Camouflaged Artificial Cells