PXD058255

PXD058255 is an original dataset announced via ProteomeXchange.

Title	Plasma proteome profiling of 37 individual samples both before and after exercise
Description	Regular voluntary exercise has been shown to increase waste transport through the glymphatic system in mice. Here, we investigated the impact of physical exercise on both upstream and downstream brain waste clearance in healthy volunteers via noninvasive MR imaging. Glymphatic influx, evaluated using intravenous contrast-enhanced dynamic T1 mapping, increased significantly at the putamen after 12 weeks of chronic exercise using a cycle ergometer (P=.01). The meningeal lymphatic vessel size and flow, measured by intravenous contrast-enhanced black-blood imaging and IR-ALADDIN technique, increased significantly after chronic exercise (P=.008 and P=.002, respectively). Plasma proteomics revealed significant changes in inflammation-related and immune-related proteins (down-regulated: S100A8, S100A9, PSMA3, and DEFA1A3; up-regulated: J chain) after chronic exercise, which correlated with glymphatic influx or mLV flow (P<.05). Our results suggest that increased glymphatic and mLV flow may be the potential mechanism underlying the neuroprotective effects of exercise on cognition, highlighting the importance of long-term, regular exercise.
HostingRepository	PRIDE
AnnounceDate	2025-04-07
AnnouncementXML	Submission_2025-04-06_23:04:17.318.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dohyun Han
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-25 16:43:10	ID requested
⏵ 1	2025-04-06 23:04:17	announced

10.1038/S41467-025-58726-1;

submitter keyword: Plasma, Glymphatic flow, exercise, mLV, Proteomics

Dohyun Han
contact affiliation	Proteomics core facility, Biomedical Research Institute, Seoul National University Hospital
contact email	hdh03@snu.ac.kr
lab head
Dohyun Han
contact affiliation	Proteomics Core Facility, Biomedical Research Institute, Seoul National University Hospital
contact email	hdh03@snu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058255
     1. Label: PRIDE project
     2. Name: Plasma proteome profiling of 37 individual samples both before and after exercise