PXD058243

PXD058243 is an original dataset announced via ProteomeXchange.

Title	Novel kinetoplast genome structure and RNA editing patterns in the trypanosomatid Vickermania
Description	The trypanosomatid flagellates possess in their single mitochondrion a highly complex kinetoplast (k) DNA, which is composed of interlocked circular molecules of two types. Dozens of maxicircles represent a classical mitochondrial genome, and thousands of minicircles encode guide (g)RNAs, which direct the processive and essential uridine insertion/deletion mRNA editing of maxicircle transcripts. While the details of kDNA structure and this type of RNA editing are well-established, our knowledge mostly relies on a narrow foray of intensely studied human parasites of the genera Leishmania and Trypanosoma. Here, we analyzed kDNA, its expression, and RNA editing of two members of the poorly characterized genus Vickermania with very different cultivation histories. In both Vickermania species, the gRNA-containing HL-circles are atypically large with multiple gRNAs each. Examination of V. spadyakhi HL-circle loci revealed a massive redundancy of gRNAs relative to the editing needs. In comparison, the HL-circle repertoire of extensively cultivated V. ingenoplastis is greatly reduced. It correlates with V. ingenoplastis-specific loss of productive editing of transcripts encoding subunits of respiratory chain complex I and corresponding lack of complex I activity. This loss in a parasite already lacking genes for subunits of complexes III and IV suggests an apparent requirement for its mitochondrial ATP synthase to work in reverse to maintain membrane potential. In contrast, V. spadyakhi retains a functional complex I that allows ATP synthase to work in its standard direction.
HostingRepository	PRIDE
AnnounceDate	2025-03-29
AnnouncementXML	Submission_2025-03-29_08:30:23.139.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058243
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Peter Barath
SpeciesList	scientific name: Vickermania; NCBI TaxID: 2795773;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-25 08:04:49	ID requested
⏵ 1	2025-03-29 08:30:23	announced

10.1073/PNAS.2426887122;

10.6019/PXD058243;

submitter keyword: Keywords

Peter Barath
contact affiliation	Institute of Chemistry, Slovak Academy of Sciences
contact email	peter.barath@savba.sk
lab head
Peter Barath
contact affiliation	Institute of Chemistry, Slovak Academy of Sciences
contact email	peter.barath@savba.sk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058243
     1. Label: PRIDE project
     2. Name: Novel kinetoplast genome structure and RNA editing patterns in the trypanosomatid Vickermania