⮝ Full datasets listing
PXD058214
PXD058214 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteome of BCG-derived membrane vesicles |
| Description | Proteins in two different BCG-derived membrane vesicles, extracellular vesicles, were compared. One is isolated from statically cultured BCG in Sauton's medium (Sample #1 and #2), commonly used to produce BCG vaccine. Another is isolated from planktonically cultured BCG in Muller-Hinton II Broth (Sample #3 and #4), commonly used for bacterial experiments. Proteins from BCG-MVs were separated via SDS-PAGE on a 12% polyacrylamide gel (10 µg in each lane). The resulting gel was stained with 10% Coomassie Brilliant Blue (G-250), and 4 pieces per lane were excised. The samples were reduced, alkylated and trypsinized via the in-gel digestion method. The peptides extracted from the gel were subjected to nanoLC-MS/MS analysis with a system consisting of an Orbitrap Velos Pro (Thermo) coupled with an Advance UHPLC (Brucker). |
| HostingRepository | jPOST |
| AnnounceDate | 2025-11-25 |
| AnnouncementXML | Submission_2025-11-25_07:00:05.768.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Takehiro Yamaguchi |
| SpeciesList | scientific name: Mycobacterium tuberculosis variant bovis BCG; NCBI TaxID: 33892; |
| ModificationList | L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine |
| Instrument | LTQ Orbitrap Velos Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-11-24 23:40:18 | ID requested | |
| ⏵ 1 | 2025-11-25 07:00:06 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: BCG, Membrane vesicles, Mycobacteria |
Contact List
| Takehiro Yamaguchi | |
|---|---|
| lab head | |
| Takehiro Yamaguchi | |
| contact affiliation | National Institute of Infectious Diseases |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003483/ |
