PXD058191

PXD058191 is an original dataset announced via ProteomeXchange.

Title	Cellular interactome dynamics during heat stress
Description	Quantitative in vivo cross-linking-mass spectrometry was used to identify significant time-dependent interactome changes that occur prior to large-scale proteome abundance remodeling in cells subjected to heat stress. Interactome changes were identified within minutes of applied heat stress, including changes in chaperone systems as expected due to altered functional demand. Global analysis of all interactome changes revealed the largest significant enrichment in the class of RNA binding proteins, providing new time-dependent conformational insight on complex relationships that exist between transcription, translation and cellular stress response mechanisms upstream of transcriptional reprogramming. Observed interactome dynamics of RNA binding proteins that are also HSP90 clients constitute mechanistic changes that increase HSP90 functional demand, evident from observed HSP90 interactome changes early in heat stress. However, these changes together with observed heat stress-induced ATP level reduction result in observed complex time-dependent HSP90 interactome dynamics, indicating HSP90 conformational bottleneck formation with increased stress duration.
HostingRepository	PRIDE
AnnounceDate	2024-11-25
AnnouncementXML	Submission_2024-11-25_13:23:57.031.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sung-Gun Park
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-22 22:16:54	ID requested
⏵ 1	2024-11-25 13:23:57	announced

Dataset with its publication pending

submitter keyword: HSP90,Interactome, Heat stress, cross-linking-mass spectrometry

James E. Bruce
contact affiliation	Department of Genome Sciences, University of Washington, Seattle
contact email	jimbruce@uw.edu
lab head
Sung-Gun Park
contact affiliation	University of Washington
contact email	sunggun20@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD058191

PRIDE project URI

• PRIDE
  1. PXD058191
     1. Label: PRIDE project
     2. Name: Cellular interactome dynamics during heat stress