PXD058184

PXD058184 is an original dataset announced via ProteomeXchange.

Title	Serine ubiquitination of SNAREs generates bacterial vacuoles and prevents their lysosomal fusion
Description	Here we show that Legionella SidE effectors mediate phosphoribosyl-linked serine ubiquitination (PR-Ub) of the autophagic SNARE proteins STX17 and SNAP29. On the one hand, PR-Ub modification of STX17 enhances its interaction with ATG14L, which drives the recruitment of ER membranes to the bacterial vacuole in a PI3K-dependent manner and promotes the formation of bacterial replicative vacuoles. On the other hand, PR-Ub of SNAP29 inhibits the formation of the autophagosomal SNARE complex (STX17-SNAP29-VAMP8) by steric hindrance and thus prevents the fusion of STX17 + bacterial vacuoles with lysosomes.
HostingRepository	PRIDE
AnnounceDate	2025-05-14
AnnouncementXML	Submission_2025-05-14_11:39:26.147.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anshu Bhattacharya
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-22 15:06:24	ID requested
⏵ 1	2025-05-14 11:39:26	announced

Dataset with its publication pending

submitter keyword: Legionella pneumophila, xenophagy, Syntaxin17, ubiquitin,Autophagy

Ivan Dikic
contact affiliation	Goethe university, Institute of Biochemistry II
contact email	dikic@biochem2.uni-frankfurt.de
lab head
Anshu Bhattacharya
contact affiliation	Denali Therapeutics
contact email	anshu.bhattacharya312@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058184

PRIDE project URI

• PRIDE
  1. PXD058184
     1. Label: PRIDE project
     2. Name: Serine ubiquitination of SNAREs generates bacterial vacuoles and prevents their lysosomal fusion