PXD058170

PXD058170 is an original dataset announced via ProteomeXchange.

Title	Proteome changes in retinal Mueller glial cells from a spontaneous equine model for autoimmune uveitis compared to healthy controls
Description	Retinal Müller glial cells (RMG) play a central role in retinal neuroinflammation, actively participating in the immune response and adopting a dynamic inflammatory phenotype. However, the precise functions of RMG in retinal inflammation remain incompletely understood. In this study, we investigated the proteome of RMG isolated from healthy horses and horses affected by equine recurrent uveitis (ERU). ERU serves as a unique spontaneous model of autoimmune uveitis, exhibiting key clinical and pathophysiological features observed in humans, such as a relapsing-remitting course of disease and a CD4+ T cell-driven autoimmune etiology [1, 2]. To gain insights into the molecular landscape of RMG in a spontaneous animal model of autoimmune uveitis and their potential contribution to retinal autoimmune neuroinflammation, we used discovery proteomics for the analysis of RMG from healthy horses and horses with ERU. Our data are not only relevant to veterinary medicine but also hold significant translational value for human ophthalmology as well. References: [1] Deeg, C.A., et al., Uveitis in horses induced by interphotoreceptor retinoid-binding protein is similar to the spontaneous disease. Eur J Immunol, 2002. 32(9): p. 2598-606. https://doi.org/10.1002/1521-4141(200209)32:9<2598::AID-IMMU2598>3.0.CO;2-%23 [2] Deeg, C.A., et al., CRALBP is a highly prevalent autoantigen for human autoimmune uveitis. Clin Dev Immunol, 2007. 2007: p. 39245. https://doi.org/10.1155/2007/39245
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_05:00:14.770.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roxane Degroote
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-22 03:57:11	ID requested
⏵ 1	2025-05-07 05:00:15	announced

10.3390/biom15020288;

Fleischer AB, Amann B, von Toerne C, Degroote RL, Schmalen A, Wei, ß, er T, Hauck SM, Deeg CA, ller Glial Cells During Autoimmune Uveitis. Biomolecules, 15(2):(2025) [pubmed]

submitter keyword: quantitative label-free LC-MS/MS

retinal neuroinflammation

autoimmune uveitis

equine recurrent uveitis (ERU)

spontaneous equine animal model

retinal Mueller glial cells (RMG)

Stefanie M Hauck
contact affiliation	Core Facility Metabolomics and Proteomics, Helmholtz Center Munich, German Research Center for Environmental Health, Munich, Germany
contact email	stefanie.hauck@helmholtz-munich.de
lab head
Roxane Degroote
contact affiliation	LMU Munich, Veterinary Sciences, Chair of Physiology, Lena-Christ-Straße 48, 82152 Planegg/Martinsried, Germany
contact email	r.degroote@lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058170

PRIDE project URI

• PRIDE
  1. PXD058170
     1. Label: PRIDE project
     2. Name: Proteome changes in retinal Mueller glial cells from a spontaneous equine model for autoimmune uveitis compared to healthy controls