PXD058168

PXD058168 is an original dataset announced via ProteomeXchange.

Title	DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts
Description	The timely termination of RNA polymerase II (Pol II) transcription is essential for recycling of this enzyme and preventing transcriptional interference. While 5'-3' exonuclease Xrn2 is known to degrade nascent RNA after poly(A) site cleavage to promote Pol II release, its molecular mechanism remains not fully understood. Spt5 is one of highly conserved transcription factors that controls and regulates multiple aspects of Pol II transcription. Here, using mass spectrometry analysis of immunoprecipitated Flag-tagged Spt5-WT and Spt5-mutant complexes from fission yeast cells, we show that Xrn2 is highly enriched in these samples. Through in vitro BS3 crosslinking experiments with purified components, we further demonstrated that Xrn2 associates with transcription machinery and we probed interfaces between different subunits of transcription machinery
HostingRepository	PRIDE
AnnounceDate	2024-11-22
AnnouncementXML	Submission_2024-11-22_04:00:57.390.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vaishnavi Ravikumar
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-22 03:45:07	ID requested
⏵ 1	2024-11-22 04:00:57	announced

Dataset with its publication pending

submitter keyword: Xrn2, MS-MS, Spt5, torpedo, fission yeast (Schizosaccharomyces pombe),DSIF, crosslinking

Lidia Vasilieva
contact affiliation	Department of Biochemistry, University of Oxford, OX13QU, Oxford, United Kingdom
contact email	lidia.vasilieva@bioch.ox.ac.uk
lab head
Vaishnavi Ravikumar
contact affiliation	Copenhagen University Hospital
contact email	vaishnavi.ravikumar@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD058168

PRIDE project URI

• PRIDE
  1. PXD058168
     1. Label: PRIDE project
     2. Name: DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts